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US 2022/0054622 A1Published application
Method of purifying virus-like particles
AssigneeThe Government of the United States of America, as represented by the Secretary of Homeland Security
InventorMichael Puckette
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Abstract
A method of producing purified FMDV VLPs, comprising contacting cells containing FMDV VLPs with a lysis buffer and allowing the cells to lyse, the lysis buffer comprising 10-20 mM Tris-HCl, 150-200 mM NaCl, 3 mM MgCl2, and 1% Triton X-100, wherein the lysis buffer does not contain EDTA; centrifuging a solution; and removing a supernatant from the solution, the supernatant containing the purified FMDV VLPs.
Claims20
A method of producing purified VLPs, comprising:
- a) contacting cells containing VLPs with a lysis buffer and allowing the cells to lyse, the lysis buffer comprising:
- 10-20 mM Tris-HCl,
- 150-200 mM NaCl,
- 3 mM MgCl2,
- and 1% Triton X-100,
- wherein the lysis buffer does not contain EDTA;
- b) centrifuging a solution of step (a);
- and
- c) removing a supernatant from the solution of step (b), the supernatant containing the purified VLPs.
6 more elements
Dependencies are inferred from the claim text.