Conditionally active receptors
AssigneeHarpoon Therapeutics, Inc.
InventorsShuoyen Jack LIN, Richard J. AUSTIN, Bryan D. LEMON, Kevin WRIGHT, Holger WESCHE
Life of this patent
Estimating the term…
Abstract
Disclosed herein are conditionally active receptors which comprise a target antigen binding domain and a binding moiety. Each binding moiety comprises non-CDR loops for masking the binding of a target antigen binding domain to its target. The receptors are activated upon cleavage of the cleavable linkers. Pharmaceutical compositions comprising cells comprising the conditionally active receptors disclosed herein and methods of using such formulations are further provided.
Claims86
A conditionally active chimeric antigen receptor that comprises a single polypeptide chain, comprising
- (a) a binding moiety comprising a non-CDR loop and a cleavable linker;
- (b) a target antigen binding domain;
- (c) a transmembrane domain;
- and
- (d) an intracellular signaling domain;
- wherein the binding moiety is capable of masking the binding of the target antigen binding domain to its target.
A conditionally active T-cell receptor fusion protein comprising a single polypeptide chain comprising:
- (a) a T-cell receptor subunit comprising:
- (i) at least a portion of a T-cell receptor extracellular domain;
- (ii) a transmembrane domain;
- and
- (iii) a T-cell receptor intracellular domain comprising a stimulatory domain from an intracellular signaling domain;
- (b) a binding moiety comprising a non-CDR loop and a cleavable linker;
- and
- (c) a target antigen binding domain;
- wherein the binding moiety is capable of masking the binding of the target antigen binding domain to its target.
A conditionally active T-cell receptor comprising a binding moiety comprising a a non-CDR loop and a cleavable linker, wherein the binding moiety is capable of masking the binding of the T-cell receptor to its target.
The conditionally active T-cell receptor of any one of claims 61-86, wherein the target antigen binding domains comprises an sdAb, a scFv, a Fab, a variable heavy chain domain (VHH), or a combination thereof.
Dependencies are inferred from the claim text.