Methods for the preparation of biologically active compounds in nanoparticulate form

The method of any preceding claim, wherein the time period is a range selected from the group consisting of between 5 minutes and 2 hours, between 5 minutes and I hour, between 5 minutes and 45 minutes, between 5 minutes and 30 minutes, and between 10 minutes and 25 minutes.

The method of any preceding claim wherein the biologically active compound is selected from the group consisting of biologics, amino acids, proteins, peptides, nucleotides, nucleic acids, and analogs, homologs and first order derivatives.

The method of any preceding claim, wherein the grinding compound is selected from the group consisting of sodium hydrogen sulfate, sodium hydrogen carbonate, sodium hydroxide, succinic acid, fumaric acid, maleic acid, tartaric acid, citric acid, ammonium chloride, methylamine hydrochloride, ammonium bromide, crystalline hydroxides, hydrogen carbonates, hydrogen carbonates of pharmaceutical acceptable alkali metals, sodium sulphate, sodium chloride, sodium metabisulphite, sodium thiosulphate, ammonium chloride, Glauber's salt, ammonium carbonate, sodium bisulphate, magnesium sulphate, potash alum, potassium chloride, sodium carbonate, sodium bicarbonate, potassium carbonate, potassium bicarbonate, and lactose.

The method of any preceding claim, further comprising the step of removing at least a portion of the at least partially milled grinding compound, wherein the nanoparticles have an average particle size of less than 200 nm.

A nanoparticle composition comprising nanoparticles of a biologically active compound, formed by the process of dry milling a solid biologically active compound and a millable grinding compound in a mill comprising a plurality of milling bodies, for a sufficient time period to produce a solid dispersion comprising nanoparticles of the biologically active compound dispersed in at least partially milled grinding compound.

A method of treating a human in need of such treatment comprising the step of administering a pharmaceutically effective amount of a member selected from the group consisting of the nanoparticle composition of any preceding claim, the pharmaceutical composition of any preceding claim, and the medicament of any preceding claim.