Method for making reduction sensitive nano micelles

A method for making reduction sensitive nano micelles comprising steps of:

• 1) dissolving taurine in distilled water in a 250 mL single port flask, adding sodium hydroxide solution and stirring in an ice water bath for 30 minutes;
• dissolving acryloyl chloride in dichloromethane to form a solution, adding the solution to the single port flask through a constant pressure funnel within 40 minutes, reacting at 25° C. for 5 hours, filtrating and washing the product with deionized water for 3 times, recrystallizing with ethyl acetate, drying in vacuum for 24 hours to obtain N-acryloyltaurine;
• 2) dissolving lipoic acid in toluene in a three-necked flask equipped with a reflux and water separation device, adding hydroxyethyl methacrylate and a catalyst, stirring for 30 minutes, reacting at 85° C. for 6 hours under protection of nitrogen, cooling and washing the product with 1.0 M sodium hydroxide solution, discarding water layer through separation, removing the toluene by vacuum distillating to obtain lipoic acid methacryloyloxyethyl ester;
• and
• 3) dissolving N-acryloyltaurine of step 1) and lipoic acid methacryloyloxyethyl ester of step 2) in dimethyl sulfoxide in a three-necked flask, stirring evenly at room temperature, adding an initiator and stirring until completely dissolved;
• ventilating nitrogen for 20 minutes, raising temperature to 60˜65° C. and reacting for 5 hours to obtain a copolymer solution, after cooling, dropping the copolymer solution slowly into deionized water, adding dithiothreitol and reacting at 25˜30° C. for 24 hours, transferring the product solution into a dialysis bag for dialysing 72 hours, to obtain reduction sensitive nano micelles after freeze-drying.