A sunflower seed protein isolate and a process for producing the same
AssigneesAvril, Universite De Lorraine, Centre National De La Recherche Scientifque
InventorsOlivier GALET, Romain KAPEL, Sara ALBE SLABI
Life of this patent
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Abstract
A process for preparing a sunflower seed protein isolate and a protein isolate which is obtainable by such process. The process comprises the following steps: mixing a defatted seed meal with an aqueous NaCl solution at a basic pH; separating said solubilised protein solution from solids; diafiltering said solubilised protein solution through an ultrafiltration membrane system using an aqueous NaCl diafiltration NaCl solution and at least 2 diavolumes of said aqueous NaCl diafiltration solution, diafiltering said NaCl-diafiltered protein; concentrating said purified protein solution; and drying said purified protein concentrate to obtain a protein isolate.
Claims17
A process for preparing a protein isolate, said protein being a sunflower seed protein, said process comprising the following steps:
- (a) providing an at least partially defatted seed meal, said seed meal being a sunflower seed meal and having a proportion of dry matter ranging from 80 wt % to 98 wt %;
- (b) mixing said at least partially defatted seed meal with an aqueous NaCl solution at a pH of about 6 to 8, in order to solubilize proteins present in said at least partially defatted seed meal and to thus obtain a solubilized protein solution, wherein said aqueous NaCl solution has a NaCl concentration ranging from 0 to 1.2 mol·L-1;
- (c) separating said solubilized protein solution from solids therein;
- (d) diafiltering said solubilized protein solution through an ultrafiltration membrane system having a molecular weight cutoff of 1 to 100 kDa, said diafiltration step being effected using:
- an aqueous NaCl diafiltration solution having a NaCl concentration ranging from 0.1 to 0.6 mol·L-1;
- and
- at least 2 diavolumes of said aqueous NaCl diafiltration solution, to obtain a NaCl-diafiltered protein solution;
- (e) subsequently to step (d), diafiltering said NaCl-diafiltered protein solution through an ultrafiltration membrane system with a molecular weight cutoff of 1 to 100 kDa, said diafiltration step (e) being effected using water, to obtain a purified protein solution;
- (f) concentrating said purified protein solution to obtain a purified protein concentrate;
- and
- (g) drying said purified protein concentrate to obtain said protein isolate;
- wherein said process does not contain a step of precipitation of said protein prior to step (d).
A sunflower seed native protein isolate, said isolate comprising sunflower globulins and albumins, said isolate having a solubility in aqueous solution which is superior, or equal, to 65% at pH 3 and superior or equal to 65% at pH 7;
- wherein said solubility at the given pH is calculated as follows:
- SoI
- p
-
- H
- =
- (
- C
- s
-
-
- x
-
-
- V
- s
- C
- i
-
-
- x
-
-
- V
- i
- )
- ×
- 100
- wherein:
- SOIpH protein solubility at given pH (%),
- Cs—protein concentration in supernatant (g·L−1),
- Vs—final volume of solution after adjustment of pH (mL),
- Ci—initial protein concentration (g·L−1),
- Vi—initial volume of solution (mL).
Dependencies are inferred from the claim text.