Methylobacterium compositions for improving corn yield
AssigneeNewLeaf Symbiotics, Inc.
InventorsNatalie Breakfield, Charles Michael McFatrich
Life of this patent
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Abstract
The present invention provides yield enhancing compositions comprising Methylobacterium, methods for improving corn yield, and methods of making the compositions. Also provided are isolated yield enhancing Methylobacterium.
Claims45
A method for improving corn plant yield that comprises:
- (a) applying a composition to a corn plant or a corn plant part, wherein the composition comprises (i) Methylobacterium isolate NLS0807 (NRRL B-67743), NLS0662 (NRRL B-67742), NLS0648 (NRRL B-67741), NLS0109 (NRRL B-67340), or variants thereof, or (ii) a combination of Methylobacterium isolate NLS0109 (NRRL B-67340) or a variant thereof, and Methylobacterium isolate NLS0017 (B-50931) or a variant thereof;
- wherein said composition further comprises at least one additional component selected from the group consisting of an additional active ingredient, an agriculturally acceptable adjuvant, and an agriculturally acceptable excipient;
- and,
- (b) growing the corn plant to maturity, thereby improving yield of the corn plant.
A corn plant or corn plant part that is coated or partially coated with a composition comprising a Methylobacterium, wherein the Methylobacterium is (i) NLS0807 (NRRL B-67743), NLS0662 (NRRL B-67742), NLS0648 (NRRL B-67741), or variants thereof, or (ii) a combination of Methylobacterium isolate NLS0109 (NRRL B-67340) or a variant thereof, and Methylobacterium isolate NLS0017 (B-50931) or a variant thereof.
A method to detect the presence in a sample of (a) Methylobacterium strain NL0807, or a variant thereof;
- (b) NLS0648 or a variant thereof;
- or (c) NLS0662 or a variant thereof, wherein said method comprises detecting the presence in the sample of a nucleic acid comprising or located within SEQ ID NO:
- 25, 26 and/or 27, SEQ ID NO:
- 37, 38 and/or 39, or SEQ ID NO:
- 49, 50 and/or 51, respectively.
The method of claim 50, wherein the detecting of the nucleic acid comprises a polymerase chain reaction, branched DNA, ligase chain reaction, transcription mediated amplification (TMA), nucleic acid sequence-based amplification (NASBA), nanopore-, mass spectroscopy, hybridization, or direct sequencing based method, or any combination thereof.
The method of claim 50, wherein said detection comprises the steps of:
- (i) contacting the sample with a DNA primer pair, wherein said primer pair, wherein said comprises forward and reverse primers for amplification of a DNA fragment comprising or located within SEQ ID NO:
- 25, 26, 27, 37, 38, 39, 49, 50 or 51, thereby generating a DNA fragment,
- (ii) contacting said DNA fragment with a probe specific for the presence of said DNA fragment, and
- (iii) comparing the results of said contacting with positive and negative controls to determine the presence of SEQ ID NO:
- 25, 26, 27, 37, 38, 39, 49, 50 or 51 in said sample.
The method of claim 50 wherein said sample is a plant material that was treated with one or more of Methylobacterium strains selected from NL0807, NLS0648, NLS0662 and variants thereof.
The method of claim 52 wherein said plant material is leaves, roots or seeds.
The method of claim 52 wherein the plant material is a processed plant product from a plant treated with one or more Methylobacterium strains selected from NL0807, NLS0648, NLS0662 and variants thereof.
The method of claim 50 wherein said sample is a soil sample.
Dependencies are inferred from the claim text.