Back to results
US 2022/0050108 A1Published application
Enzyme quantification
AssigneeBio-Rad Laboratories, Inc.
InventorsDarren R. Link, Michael L. Samuels
Life of this patent
Estimating the term…
Abstract
The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.
Claims21
1-18. (canceled)
A method for detecting target molecules, the method comprising:
- providing a fluid sample comprising biological cells and a detection component for binding to target molecules secreted from the biological cells;
- partitioning the fluid sample into a plurality of separate and isolated partitions of fluid;
- and
- detecting each of the plurality of partitions associated with one or more secreted target molecules of one or more of the plurality of partitions, wherein the one or more secreted target molecules is a protein.
1 more element
Dependencies are inferred from the claim text.