Chromatin mapping assays and kits using long-read sequencing
AssigneeEpiCypher, Inc.
InventorsZu-Wen Sun, Martis W. Cowles, Michael-Christopher Keogh, Ellen N. Weinzapfel
Life of this patent
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Abstract
This present invention relates to methods for carrying out chromatin mapping assays that use enzymes to incorporate barcoded DNA at targeted genomic regions followed by long-read sequencing (e.g., Third Generation Sequencing (TGS)). This approach enables the mapping of chromatin targets using TGS and can be used for a wide range of elements or features, including histone post-translational modifications, chromatin associated proteins, nucleosome positioning, and chromatin accessibility. The invention further relates to kits and reagents for carrying out the methods on chromatin samples that include one or more cells.
Claims34
A synthetic transposon comprising a DNA barcode region linked on its 5′ and 3′ end to a flanking region that is recognized by a transposase, wherein the synthetic transposon does not encode a transposase.
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A method for chromatin mapping, comprising:
- a) targeting an enzyme to a specific feature in chromatin in a sample;
- b) activating the enzyme to alter or label DNA local to the feature;
- c) preparing the chromatin for sequencing;
- d) sequencing the chromatin using long-read sequencing;
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- e) mapping the location of the chromatin feature based on the locations of altered or labeled DNA.
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Dependencies are inferred from the claim text.