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US 2022/0042995 A1Published application
Enzyme quantification
AssigneeBio-Rad Laboratories, Inc.
InventorsDarren R. Link, Michael L. Samuels
Life of this patent
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Abstract
The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.
Claims21
1-18. (canceled)
A method for detecting target molecules in a fluid sample, the method comprising:
- providing, to a microfluidic device, a fluid sample comprising target molecules, a plurality of beads each comprising a reactive component for binding a target molecule thereto, and a detection component for binding to a target molecule that is bound to a bead;
- partitioning, in the microfluidic device, the fluid sample into plurality of separate and isolated partitions of fluid;
- and
- monitoring each of the plurality of partitions for detection of an event associated with contents of one or more of the plurality of partitions.
1 more element
Dependencies are inferred from the claim text.