Protein Solubility Screening Kits and Their Use
AssigneeProStabilis, Inc.
InventorsRamil Latypov, Yongwu Yang
Life of this patent
Estimating the term…
Abstract
The present invention provides a multitude of biomacromolecule solubility screening kits, and methods of using such kits. Such kits provide a substantial improvement over presently available kits and methods and provide a substantial decrease in the amounts of biomacromolecules required to run such solubility screening.
Claims18
A kit for the screening of biomacromolecule solubility comprising:
- deposited in the wells of a microplate, at least one range of core pH conditions, such range having sufficient pH variability to evaluate solubility of such biomacromolecules.
A kit for the screening of biomacromolecule solubility comprising:
- a. deposited in the wells of a microplate, at least one range of core pH conditions, such range having sufficient pH variability to evaluate solubility of such biomacromolecules;
- and
- b. at least one crowding agent, having at least one concentration of such at least one crowding agent, wherein each such concentration of crowding agent being overlayed on at least one range of core pH conditions.
A kit for screening of biomacromolecule solubility comprising:
- a. deposited in the wells of a microplate, at least one range of core pH conditions, such range having sufficient pH variability to evaluate solubility of such biomacromolecules;
- and
- b. at least one crowding agent, having at least one concentration of such at least one crowding agent, wherein each such concentration of crowding agent being overlayed on at least one range of core pH conditions, and at least one tonicity modifying agent, wherein such tonicity modifying agent being overlayed on at least one range of core pH conditions and across each of the at least one concentration of such crowding agent.
A kit for screening the solubility of biomacromolecules comprising:
- a. deposited in the wells of a microplate, at least one range of core pH conditions, such range having sufficient pH variability to evaluate solubility of such biomacromolecules;
- and
- b. at least one crowding agent, having at least one concentration of such at least one crowding agent, wherein each such concentration of crowding agent being overlayed on at least one range of core pH conditions, and at least one additional different millimolar concentration of the same buffers being overlayed on at least one range of core pH conditions and across each of the at least one concentration of such crowding agent.
A kit for screening the solubility of biomacromolecules comprising:
- a. deposited in the wells of a microplate, at least one range of core pH conditions, such range having sufficient pH variability to evaluate solubility of such biomacromolecules;
- and
- b. at least one crowding agent, having at least one concentration of such at least one crowding agent, wherein each such concentration of crowding agent being overlayed on at least one range of core pH conditions, and at least one of the compounds selected from the group consisting of an amino acid, a sugar, and combinations thereof, each such selected amino acid, sugar, and combination thereof being overlayed on at least one range of core pH conditions and across each of the at least one concentration of such crowding agent.
A kit for screening the solubility of biomacromolecules comprising:
- a. deposited in the wells of a microplate, at least one range of core pH conditions, such range having sufficient pH variability to evaluate solubility of such biomacromolecules;
- and
- b. at least one crowding agent, having at least one concentration of such at least one crowding agent, wherein each such concentration of crowding agent being overlayed on at least one range of core pH conditions, and at least one of the compounds selected from the group consisting of a cyclodextrin and a surfactant, each such selected cyclodextrin and surfactant being overlayed on at least one range of core pH conditions and across the at least one concentration of such crowding agent.
Dependencies are inferred from the claim text.