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US 2022/0042969 A1Published application
Mass control system for chromatography
AssigneeC Technologies AB
InventorsCraig Harrison, Ramsey Shanbaky
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Abstract
The present invention relates to methods for controlling chromatographic processes in real-time via mass measurement utilizing a variable pathlength spectrophotometer.
Claims21
A method for determining the breakthrough percentage of a chromatography column having an inlet and an outlet comprising:
- determining an initial slope (m0) by flowing a harvested cell culture fluid through the chromatography column for enough time to establish a signal that is not changing wherein the initial slope is determine by slope spectroscopy;
- determining a first slope (m1) by slope spectroscopy with a first sensor positioned at the inlet to the chromatography column;
- determining a second slope (m2) by slope spectroscopy with a second sensor positioned at the outlet to the chromatography column;
- and
- determining the breakthrough percentage by calculating % BT=(m2−m0)/(m1−m0)*100.
2 more elements
2-4. (canceled)
A method for determining a protein titer of a chromatography column having an inlet and an outlet comprising:
- determining an initial slope (m0) by flowing a harvested cell culture fluid through the chromatography column for enough time to establish a signal that is not changing wherein the initial slope is determine by slope spectroscopy;
- determining a first slope (m1) by positioning a sensor at the inlet to the column and measuring the slope by slope spectroscopy;
- and
- determining a titer of the chromatography column by calculating Titer=(m1−m0)/EC wherein EC is an extinction coefficient of a protein in units of mL/mg*cm.
1 more element
Dependencies are inferred from the claim text.