Production method for rare fatty acid using novel enzyme, and novel rare fatty acid
AssigneesKyoto University, Noster Inc.
InventorsJun OGAWA, Shigenobu KISHINO, Kohey KITAO
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Abstract
A method for producing a hydroxylated fatty acid by a hydration reaction using a novel enzyme derived from the genus Clostridium in the soil and using fatty acid as a substrate is provided by the present invention. In addition, a novel rare fatty acid with utility value which is obtained by the production method is also provided.
Claims22
An isolated enzyme protein of any of the following (a)-(c):
- (a) an enzyme protein consisting of the amino acid sequence shown in SEQ ID NO:
- 2,
- (b) a protein comprising an amino acid sequence resulting from deletion and/or substitution and/or insertion and/or addition of one or plural amino acids in the amino acid sequence shown in SEQ ID NO:
- 2, and having an enzyme activity to catalyze a hydration reaction,
- (c) a protein encoded by a base sequence that hybridizes to a nucleic acid consisting of a complementary chain sequence of the base sequence shown in SEQ ID NO:
- 1 under stringent conditions, and having an enzyme activity to catalyze a hydration reaction.
A hydroxylated or oxo fatty acid selected from the group consisting of 11-hydroxy-cis-4,cis-7,cis-13,cis-16,cis-19-docosapentaenoic acid, 11-hydroxy-cis-7,cis-13,cis-16,cis-19-docosatetraenoic acid, 12-hydroxy-cis-5,cis-8,cis-14,cis-17-eicosatetraenoic acid, 11-oxo-cis-4,cis-7,cis-13,cis-16,cis-19-docosapentaenoic acid, 11-oxo-cis-7,cis-13,cis-16,cis-19-docosatetraenoic acid, and 12-oxo-cis-5,cis-8,cis-14,cis-17-eicosatetraenoic acid.
-19. (canceled)
-25. (canceled)
Dependencies are inferred from the claim text.