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US 2022/0042027 A1Published application
Fruit-specific promoters
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsJames G. Thomson, Roger L. Thilmony, Kasturi Dasgupta, Christopher D. Dardick
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Abstract
The present disclosure provides genetic constructs containing a promotor that is useful in driving fruit-specific expression in plants. Further provided are expression vectors, transgenic plants, and plant parts containing such genetic constructs, as well as uses thereof.
Claims13
1:
- A genetic construct comprising a promoter operably linked to a heterologous nucleotide sequence encoding a product of interest, wherein the promoter comprises a sequence selected from the group consisting of SEQ ID NOs:
- 2-5, or a sequence having at least 90% identity thereto.
2:
- The genetic construct of claim 1, wherein the product of interest is an RNA molecule or a polypeptide.
3:
- The genetic construct of claim 1, wherein the product of interest is in a metabolic pathway selected from the group consisting of an anthocyanin metabolic pathway, a tocopherol metabolic pathway, a fatty acid metabolic pathway, a carotenoid metabolic pathway, a lycopene metabolic pathway, a betalain metabolic pathway, and a flavonoid metabolic pathway.
4:
- The genetic construct of claim 1, wherein the product of interest is a polypeptide selected from the group consisting of a MYB transcription factor, a phytoene synthase (PSY), a lycopene cyclase (LCY), and a DXP synthase (DXS).
5:
- An expression vector comprising the genetic construct of claim 1.
6:
- A transgenic plant comprising the genetic construct of claim 1.
7:
- A plant part from the transgenic plant of claim 6, wherein the plant part comprises the genetic construct.
8:
- The plant part of claim 7, wherein the plant part is a stem, a branch, a root, a leaf, a flower, a fruit, a seed, a cutting, a bud, a cell, or a portion thereof.
9:
- A method for modifying a fruit phenotype in a plant, comprising:
- (i) transforming a plant cell with the genetic construct of claim 1, wherein expression of the product of interest is associated with modification of the fruit phenotype;
- (ii) regenerating a plant from the transformed plant cell;
- and
- (iii) growing the regenerated plant to produce fruit of the modified phenotype.
2 more elements
10:
- The method of claim 9, wherein the fruit phenotype is selected from the group consisting of size, weight, color, shape, firmness, glossiness, flavor, aroma, secondary metabolite content, peel thickness, seed number, juice quality, juice sugar content, juice acid content, juice taste, juice color, and juice yield.
11:
- The method of claim 9, wherein the fruit phenotype is selected from the group consisting of anthocyanin content, tocopherol content, fatty acid content, carotenoid content, lycopene content, betalain content, and flavonoid content.
12:
- The method of claim 9, wherein the plant is selected from the group consisting of orange (Citrus sinensis), mandarin (Citrus reticulata), lime (Citrus aurantifolia), grapefruit (Citrus paradisi), lemon (Citrus ziman), pomelo (Citrus maxima), citron (Citrus medica), papeda (Citrus micrantha), and Prunus sp.
13:
- A method for creating a tomato plant with seedless fruit, comprising:
- (i) transforming a tomato plant cell with the genetic construct of claim 1, wherein the promoter comprises the sequence of SEQ ID NO:
- 4, or a sequence having at least 90% identity thereto;
- (ii) regenerating a tomato plant from the transformed tomato plant cell;
- and
- (iii) growing the regenerated tomato plant to produce seedless fruit.
3 more elements