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AssigneeZhejiang Senwei Biomedical Development Co., Ltd
InventorsDongsheng Dai, Xun Li, Xin Tian
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The present invention relates to a new method for rescuing an influenza virus and a composition therefor. The method comprises providing a host cell stably integrated with and expressing influenza virus PA, PB1, PB2 and NP genes, and introducing an influenza virus rescue system in which a stop codon is introduced into the PA, PB1, PB2 and NP genes respectively into the host cell to achieve virus rescue. The produced virus particles can be used as a live attenuated influenza vaccine, which is characterized in that, since the genes encoding the related proteins are mutated, it has no replication and proliferation ability in human and normal animal cells, and replication and proliferation can be achieved only in the host cells constructed above and it can fully stimulate the body immunity and effectively protect the body while ensuring the safety.
A method for rescuing an influenza virus, characterized in that a mammalian host cell stably expressing influenza virus PA, PB1, PB2 and NP genes is provided, and an influenza virus rescue system comprising mutant PA, PB1, PB2 and NP genes is introduced into the aforementioned host cell to achieve rescue, wherein the mutations make the influenza virus rescue system unable to rescue intact virus in natural mammalian cells.
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