Multiplexed spectral lifetime detection of phosphors
AssigneesIntelligent Material Solutions, Inc., Leiden University Medical Center, The U.S.A., as represented by the Secretary, Department of Health and Human Services
InventorsHoward Y. BELL, Joshua E. Collins, Paul L.A.M. Corstjens, Sukwan Handali, Hans J. Tanke
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Abstract
New methods and assays for multiplexed detection of analytes using phosphors that are uniform in morphology, size, and composition based on their unique optical lifetime signatures are described herein. The described assays and methods can be used for imaging or detection of multiple unique chemical or biological markers simultaneously in a single assay readout.
Claims11
A method for detecting an analyte in a sample, comprising the steps of:
- (a) contacting the sample with a phosphor particle, wherein the phosphor particle is conjugated to a capture molecule specific for an analyte of interest, to bind and label the analyte of interest;
- and
- (b) detecting the labelled analyte by the unique optical lifetime signature of the phosphor particle;
- wherein the phosphor particle conjugated to the capture molecule has a unique optical lifetime signature and uniform morphology, size, and/or composition.
An assay kit for detecting an analyte in a sample, comprising a phosphor particle conjugated to a capture molecule specific for the analyte, wherein the phosphor particle conjugated to the capture molecule has a unique optical lifetime signature and uniform morphology, size, and/or composition.
Dependencies are inferred from the claim text.