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OwnerJonathan Luke HEENEY, Simon FROST +4inventors
InventorsJonathan Luke HEENEY, Simon FROST, Ralf WAGNER, Benedikt ASBACH, Rebecca KINSLEY, Edward WRIGHT
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Methods for identifying optimized antigenic pathogen polypeptides capable of inducing a broadly neutralizing immune response, and associated T-cell responses, to a pathogen are described, as well as nucleic acid sequences encoding such polypeptides. Methods for determining whether a broadly neutralizing immune response is induced in a subject following immunization with an optimized antigenic pathogen polypeptide, or a nucleic acid encoding the optimized pathogen polypeptide, are also described. Nucleic acid molecules, polypeptides, vectors, cells, fusion proteins, pharmaceutical compositions, and their use as vaccines against pathogens, especially against emerging or re-emerging pathogens (particularly RNA viruses), are also described.
A method for identifying a lead candidate optimized antigenic pathogen polypeptide capable of inducing a broadly neutralizing immune response to a pathogen, which comprises:
A method according to any preceding claim, wherein the candidate optimized antigenic pathogen polypeptides of the polypeptide library have been expressed in, or on the surface of, mammalian cells.
A method according to any preceding claim, wherein the pathogen is a virus, the candidate optimized antigenic pathogen polypeptides are candidate optimized antigenic virus polypeptides, and the pathogen peptides are virus polypeptides.
A method according to any preceding claim, which further comprises generating the polypeptide library.
A method according to any preceding claim, wherein the different pathogen isolates include different pathogen isolates from an outbreak of a pathogen of the same subtype as the pathogen to which it is desired to induce a broadly neutralizing immune response.
A method according to any preceding claim, wherein the different pathogen isolates include different pathogen isolates from an outbreak of a pathogen of a different subtype, but the same type, as the pathogen to which it is desired to induce a broadly neutralizing immune response.
A method according to any preceding claim, wherein the different pathogen isolates include different pathogen isolates from an outbreak of a pathogen of a different group, but the same family, as the pathogen to which it is desired to induce a broadly neutralizing immune response.
A method according to any preceding claim, wherein the different pathogen isolates include different prior pathogen isolates of a pathogen of the same subtype, type, or family as the pathogen to which it is desired to induce a broadly neutralizing immune response.
A method according to any preceding claim, wherein each candidate optimized antigenic pathogen polypeptide comprises at least 20 amino acid residues.
A method according to any preceding claim, wherein the pathogen is a virus.
A method according to any preceding claim, wherein the pathogen polypeptide is a viral glycoprotein.
A method according to any preceding claim, which is an in vitro method.
A method of identifying a nucleic acid sequence encoding an optimized antigenic pathogen polypeptide capable of inducing a broadly neutralizing immune response to a pathogen, which comprises:
An isolated nucleic acid molecule, comprising a nucleic acid sequence that is:
An isolated nucleic acid molecule, comprising a nucleic acid sequence that is:
An isolated nucleic acid molecule, comprising a nucleic acid sequence that is:
An isolated polypeptide, comprising an amino acid sequence that is:
An isolated polypeptide, comprising an amino acid sequence that is:
An isolated polypeptide, comprising an amino acid sequence that is:
A nucleic acid expression vector, which comprises a multiple cloning site, comprising KpnI and NotI endonuclease sites.
An isolated nucleic acid molecule which comprises a nucleotide sequence encoding a polypeptide comprising an amino acid sequence of SEQ ID NO:
An isolated nucleic acid molecule which comprises a nucleotide sequence encoding a polypeptide comprising an amino acid sequence of SEQ ID NO:
A composition comprising a first nucleic acid which includes a nucleotide sequence encoding a polypeptide comprising an amino acid sequence of SEQ ID NO:
A composition comprising a first nucleic acid which includes a nucleotide sequence encoding a polypeptide comprising an amino acid sequence of SEQ ID NO:
A combined preparation comprising:
A combined preparation comprising:
A composition comprising a first polypeptide comprising an amino acid sequence of SEQ ID NO:
A composition comprising a first polypeptide comprising an amino acid sequence of SEQ ID NO:
A fusion protein comprising a first polypeptide comprising an amino acid sequence of SEQ ID NO:
A fusion protein comprising a first polypeptide comprising an amino acid sequence of SEQ ID NO:
A combined preparation comprising:
A combined preparation comprising:
Dependencies are inferred from the claim text.