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US 2022/0034897 A1Published application
Assays for detecting modified compounds
AssigneeEnzo Life Sciences, Inc.
InventorsElazar Rabbani, Jannis G. Stavrianopoulos, Joshua Rabbani, Praveen Pande
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Abstract
Provided are methods and compositions which are useful for separating, isolating, detecting, and quantifying compounds of interest which have been modified chemically, enzymatically or catalytically from other compounds which have not been so modified. The modifications may take the form of functional groups which are gained, lost or retained by the compounds of interest.
Claims8
A method for detecting or quantifying an enzyme capable of adding a functional group to a compound in a sample, comprising the steps of:
- (a) providing:
- (i) a compound which can gain a functional group from an enzyme, the compound comprising at least one non-radioactive signaling moiety;
- (ii) a sample suspected of containing an enzyme that could add a functional group to the compound;
- and
- (b) forming a mixture comprising the compound and the sample;
- and
- (c) separating any compound that has gained the functional group;
- and
- (d) detecting or quantifying the non-radioactive signaling moiety of any separated compounds having the functional group, thereby detecting or quantifying the enzyme in the sample.
6 more elements
Dependencies are inferred from the claim text.