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US 2022/0033891 A1Published application
Amplification with primers of limited nucleotide composition
AssigneeAtila Biosystems, Inc.
InventorsYouxiang Wang, Zhijie Yang, Xin Chen
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Abstract
The invention provides methods of amplification from a single primer or a pair of forward and reverse primers of limited nucleotide composition. Limited nucleotide composition means that the primers are underrepresented in at least one nucleotide type. Such primers have much reduced capacity to prime from each other or to extend initiated by mispriming from other than at their intended primer binding sites in a target nucleic acid.
Claims21
A method of amplifying a segment of a target nucleic acid comprising:
- contacting a sample comprising a target nucleic acid with forward and reverse primers;
- and
- conducting an amplification reaction wherein an amplified segment of the target nucleic acid is formed by extension of the forward and reverse primers with the target nucleic acid serving as a template;
- wherein
- the primers are underrepresented in one or more of the four standard nucleotide types, the underrepresented nucleotide type(s) being the same in the primers, and the amplified segment is the predominant amplification product formed from by extension of the forward and/or reverse primers.
2 more elements
21-84. (canceled)
Dependencies are inferred from the claim text.