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US 2022/0033861 A1Published application
Solubilization of msw with blend enzymes
AssigneeRenescience A/S
InventorsHanne Risbjerg Soerensen, Lisa Rosgaard, Henrik B. Nielsen, Lone Baekgaard, Joanna Wawrzynczyk
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Abstract
The present invention relates to a method for solubilisation or hydrolysis of Municipal Solid Waste (MSW) with an enzyme blend and an enzyme composition for solubilization of Municipal Solid Waste (MSW), the enzyme composition comprising a cellulolytic background composition and a protease, lipase and/or beta-glucanase.
Claims29
A process for solubilizing waste comprising:
- contacting Municipal Solid Waste (MSW) with an enzyme composition comprising a cellulolytic background composition comprising (a) a cellobiohydrolase I or variant thereof;
- (b) a cellobiohydrolase II or variant thereof;
- (c) a beta-glucosidase or variant thereof;
- and (d) a polypeptide having cellulolytic enhancing activity;
- and one or more enzymes selected from (i) a protease;
- (ii) a lipase and (iii) a beta-glucanase.
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A process for solubilizing waste comprising:
- contacting Municipal Solid Waste (MSW) with an enzyme composition comprising a cellulolytic background composition comprising (a) a cellobiohydrolase I or variant thereof;
- (b) a cellobiohydrolase II or variant thereof;
- (c) a beta-glucosidase or variant thereof;
- and (d) a polypeptide having cellulolytic enhancing activity;
- and further comprising a beta-glucanase, wherein the beta-glucanase of the enzyme composition is present at a ratio between about 1-30% w/w, between about 10-20% w/w or about 15% w/w of the total enzyme protein,
- wherein the amount of enzyme composition to MSW is about 0.5 to about 50 mg per gram of MSW.
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Dependencies are inferred from the claim text.