# US 2022/0033799 A1 · Polypeptides having Peptidoglycan Degrading Activity and Polynucleotides Encoding Same > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2022/0033799 A1 - Document ID: US20220033799A1 - Status: Published application - Kind: A1 - Application number: 17/298,836 - Filed: 2019-12-19 - Published: 2022-02-03 - Earliest claimed: 2018-12-21 - Assignees: Novozymes A/S - Applicants: Novozymes A/S - Inventors: Christian Berg Oehlenschlaeger; Dorotea Raventos Segura; Jesper Salomon; Fabian Barrientos Garcia; Lillian Eva Tang Baltsen; Christian Bech Rosen - Web page: https://patentgrep.com/patent/US20220033799A1 ## CPC - C12N 9/80: acting on amide bonds in linear amides {(3.5.1)} - C11D 3/38636: {containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase} - C12Y 305/01028: N-Acetylmuramoyl-L-alanine amidase (3.5.1.28) - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - C11D: DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL - C12Y: ENZYMES ## Abstract The present invention relates to cleaning compositions comprising polypeptides having peptidoglycan degradation activity, as well as use of the cleaning compositions for cleaning of an item such as a textile or a surface. ## Claims ### Claim 1 A cleaning composition comprising a peptidoglycan degradation enzyme, at least one surfactant, and at least one additional cleaning component selected from builders and bleach components. ### Claim 2 (depends on 1) The cleaning composition of claim 1, comprising a peptidoglycan degradation enzyme, at least 5 wt % anionic surfactants, and at least one additional cleaning component selected from at least one builder and at least one bleach component. ### Claim 3 (depends on 1) The cleaning composition according to claim 1, wherein the peptidoglycan degrading enzyme has N-acetylmuramyl-L-alanine amidase activity. ### Claim 4 (depends on 1) The cleaning composition according to claim 1, wherein the peptidoglycan degrading enzyme has peptidoglycan lyase activity. ### Claim 5 (depends on 3) The cleaning composition according to claim 3, or wherein the peptidoglycan degradation enzyme has N-acetylmuramyl-L-alanine amidase activity and peptidoglycan lyase activity. ### Claim 6 (depends on 1) The cleaning composition according to claim 1, wherein the peptidoglycan degradation enzyme comprises the motif N[IV]X[AG][GAS]A[AY][LV]L (SEQ ID NO: 111). ### Claim 7 (depends on 6) The cleaning composition of claim 6, wherein the peptidoglycan degradation enzyme is selected from the group consisting of: SEQ ID NO: 3, SEQ ID NO: 6, SEQ ID NO: 9, SEQ ID NO: 12, SEQ ID NO: 15, SEQ ID NO: 18, SEQ ID NO: 21, SEQ ID NO: 24, SEQ ID NO: 27, SEQ ID NO: 42, SEQ ID NO: 45, SEQ ID NO: 48, SEQ ID NO: 51, SEQ ID NO: 54, SEQ ID NO: 60, SEQ ID NO: 63, SEQ ID NO: 66, SEQ ID NO: 69, SEQ ID NO: 75, SEQ ID NO: 87, SEQ ID NO: 96, SEQ ID NO: 99 and SEQ ID NO: 108, and polypeptides having at least at least 60% sequence identity thereto, and wherein the enzyme has peptidoglycan degradation activity. ### Claim 8 (depends on 1) A cleaning composition according to claim 1, comprising about 5 to about 60 wt % of at least one surfactant, and further comprising: a. about 5 wt % to about 50 wt % of at least one builder; and/or b. about 1 wt % to about 20 wt % of at least one bleach component. ### Claim 9 (depends on 1) A cleaning composition according to claim 1, wherein the polypeptide having peptidoglycan degradation activity is selected from the group consisting of: i. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 3, ii. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 6, iii. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 9, iv. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 12, v. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 15, vi. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 18, vii. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 21, viii. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 24, ix. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 27, x. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 30, xi. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 33, xii. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 36, xiii. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 39, xiv. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 42, xv. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 45, xvi. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 48, xvii. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 51, xviii. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 54, xix. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 57, xx. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 60, xxi. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 63, xxii. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 66, xxiii. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 69, xxiv. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 72, xxv. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 75, xxvi. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 78, xxvii. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 81, xxviii. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 84, xxix. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 87 xxx. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 90, xxxi. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 93, xxxii. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 96, xxxiii. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 99, xxxiv. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 102, xxxv. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 105, and xxxvi. a polypeptide having at least at least 60% sequence identity to SEQ ID NO: 108. ### Claim 10 (depends on 9) The cleaning composition according to claim 9, wherein the polypeptide has N-acetylmuramyl-L-alanine amidase and peptidoglycan lyase activity. ### Claim at position 11 11-12. (canceled) ### Claim 13 A method of cleaning on an item, comprising: a) contacting the item with a solution comprising a peptidoglycan degradation enzyme having peptidoglycan lyase activity and N-acetylmuramyl-L-alanine amidase activity, and a cleaning component, wherein the cleaning component is selected from 5 to 60 wt % of at least one surfactant; 5 to 50 wt % of at least one builder; and 1 to 20 wt % of at least one bleach component, and optionally b) rinsing the item; wherein the item is a textile.