# US 2022/0033768 A1 · Living mammalian cells modified with functional modular nanoparticles

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- Number: US 2022/0033768 A1
- Document ID: US20220033768A1
- Status: Published application
- Kind: A1
- Application number: 17/277,256
- Filed: 2019-09-24
- Published: 2022-02-03
- Earliest claimed: 2018-09-24
- Assignees: UNM Rainforest Innovations
- Inventors: Jimin Guo; C. Jeffrey Brinker; Wei Zhu; Jacob Ongudi Agola
- Web page: https://patentgrep.com/patent/US20220033768A1

## CPC

- C12N 5/0012: {Cell encapsulation}
- C12N 5/0602: {Vertebrate cells}
- B82Y 5/00: Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
- B82Y 30/00: Nanotechnology for materials or surface science, e.g. nanocomposites
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- B82Y: SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES

## Abstract

An encapsulated living (viable) mammalian cell, and methods of making and using that cell, are provided.

## Claims

### Claim 1

A viable mammalian cell which comprises a plurality of linked nanoparticles enveloping the cell.

### Claim 2 (depends on 1)

The mammalian cell of claim 1 wherein the cell is a human cell.

### Claim 3 (depends on 1)

The mammalian cell of claim 1 wherein an individual nanoparticle has a diameter of about 5 nm to about 500 nm, about 10 nm to about 300 nm or about 15 nm to about 250 nm.

### Claim at position 4

4-5. (canceled)

### Claim 6 (depends on 1)

The mammalian cell of claim 1 wherein the nanoparticles are metal-organic nanoparticles.

### Claim 7 (depends on 6)

The mammalian cell of claim 6 wherein the nanoparticles comprise Zn, Fe, Zr, or Co or iron oxide.

### Claim 8

(canceled)

### Claim 9 (depends on 1)

The mammalian cell of claim 1 wherein the nanoparticles are linked via a metal-phenolic interaction, a boronic acid-phenolic acid interaction or via a thiol linkage.

### Claim 10

(canceled)

### Claim 11 (depends on 1)

The mammalian cell of claim 1 wherein the nanoparticles comprise silica.

### Claim 12

(canceled)

### Claim 13 (depends on 1)

The mammalian cell of claim 1 wherein the nanoparticles are functionalized with amine or phenol prior to linking.

### Claim 14

(canceled)

### Claim 15 (depends on 1)

The mammalian cell of claim 1 wherein the linkage is reversible.

### Claim 16 (depends on 15)

The mammalian cell of claim 15 wherein the linkage is reversible with a metal chelator.

### Claim 17 (depends on 1)

The mammalian cell of claim 1 wherein a molecule having a diameter of about 5 nm or greater cannot permeate the linked nanoparticles.

### Claim at position 17

18-19. (canceled)

### Claim 20 (depends on 1)

The mammalian cell of claim 1 wherein a molecule having a diameter of less than about 3 nm can permeate the linked nanoparticles.

### Claim 21

(canceled)

### Claim 22

A method of preparing a viable mammalian cell the circumference of which is surrounded by a plurality of linked nanoparticles, comprising:
combining an amount of a plurality of viable mammalian cells and an amount of a plurality of nanoparticles which are functionalized with one or more linkers under conditions that result in one or more mammalian cells being encapsulated with a plurality of linked nanoparticles.

### Claim 23

A method of preparing a viable mammalian cell the circumference of which is surrounded by a plurality of linked nanoparticles, comprising:
combining an amount of a plurality of viable mammalian cells, an amount of a plurality of nanoparticles, and an amount of linkers under conditions that result in one or more mammalian cells being encapsulated with a plurality of linked nanoparticles.

### Claim 24 (depends on 22)

The method of claim 22 wherein the linkage is reversible.

### Claim 25 (depends on 22)

The method of claim 22 wherein the encapsulated mammalian cells have sensing, electrical or magnetic properties.

### Claim 26 (depends on 22)

The method of claim 22 wherein the cells are human cells.

### Claim 27

(canceled)

### Claim 28 (depends on 22)

The method of claim 22 wherein the nanoparticles are metal-organic nanoparticles or comprise silica or iron oxide.

### Claim at position 27

29-30. (canceled)

### Claim 31 (depends on 22)

The method of claim 22 wherein the nanoparticles are linked via a metal-phenolic interaction, via a boronic acid-phenolic acid interaction or via a thiol linkage.

### Claim at position 29

32-34. (canceled)

### Claim 35 (depends on 22)

The method of claim 22 wherein the encapsulated cells have enhanced viability in response to alterations in pH, alterations in ROS levels, osmotic stress or UV exposure or are optically detectable.

### Claim 36

(canceled)