# US 2022/0033752 A1 · Bioreactor for cell culture > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2022/0033752 A1 - Document ID: US20220033752A1 - Status: Published application - Kind: A1 - Application number: 17/100,972 - Filed: 2020-11-23 - Published: 2022-02-03 - Earliest claimed: 2020-07-31 - Assignees: Prakashkumar Narasimhamurthy - Inventors: Prakashkumar Narasimhamurthy; Yougunn Prakashkumar; Saradha Prakashkumar - Web page: https://patentgrep.com/patent/US20220033752A1 ## CPC - C12M 23/58: {Reaction vessels connected in series or in parallel (combinations of bioreactors with other apparatus, C12M43/00)} - C12M 25/02: {Membranes; Filters (filters or filtration in general B01D24/00-B01D41/00)} - C12M 23/14: {Bags} - C12M 23/34: {Internal compartments or partitions} - C12M 23/42: {Integrated assemblies, e.g. cassettes or cartridges} - C12M 23/44: {Multiple separable units; Modules} - C12M 25/14: {Scaffolds; Matrices (in general C12N5/0068)} - C12M 25/18: {Fixed or packed bed} - C12M 27/18: {Flow directing inserts} - C12M 27/20: {Baffles; Ribs; Ribbons; Auger vanes} - C12M 29/06: {Nozzles; Sprayers; Spargers; Diffusers (per se B01F23/231, B01J19/26)} - C12M 29/18: {External loop; Means for reintroduction of fermented biomass or liquid percolate (loop type reactors for chemical or physical processes B01J19/2435)} - C12M 33/22: {Settling tanks; Sedimentation by gravity (settling tanks per se B01D21/02)} - C12M 41/32: {of substances in solution} - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} ## Abstract A bioreactor for cell culture comprising, a first set of open top troughs that are interconnected by a first set off low channels to form a first cascade, a second set of open top troughs that are interconnected by a second set of flow channels to form a second cascade, and a set of interconnecting flow channels coupled between the first cascade and the second cascade, wherein the first cascade circulates a fresh media and the second cascade circulates a cell culture and a first quantity of the fresh media from first cascade is supplied to the second cascade through the set of interconnecting flow channels when a second quantity of cell culture is removed from the second cascade. ## Claims ### Claim 1 A bioreactor for cell culture comprising: a first set of open top troughs that are interconnected by a first set of flow channels to form a first cascade; a second set of open top troughs that are interconnected by a second set of flow channels to form a second cascade; and a set of interconnecting flow channels coupled between the first cascade and the second cascade, wherein the first cascade circulates a fresh media and the second cascade circulates a cell culture and a first quantity of the fresh media from first cascade is supplied to the second cascade through the set of interconnecting flow channels when a second quantity of cell culture is removed from the second cascade. ### Claim 2 (depends on 1) The bioreactor of claim 1, wherein the first and the second set of flow channels are inclined open top channels comprising at least one of a plurality of descending steps with openings between the steps and a gas permeable base. ### Claim 3 (depends on 1) The bioreactor of claim 1, wherein the set of interconnecting flow channels are inclined and closed hollow tubes allowing a free flow of the fresh media from the first cascade to the second cascade. ### Claim 4 (depends on 1) The bioreactor of claim 1, wherein the first set of flow channels are correspondingly coupled between a first set of overflow outlets in the first set of open top troughs and a first set of inlets in the next of the first set of open top troughs to form the first cascade. ### Claim 5 (depends on 4) The bioreactor of claim 4, wherein the second set of flow channels are correspondingly coupled between a second set of overflow outlets in the second set of open top troughs and a second set of inlets in the next of the second set of open top troughs to form the second cascade. ### Claim 6 (depends on 5) The bioreactor of claim 5, wherein the first cascade, the second cascade and the set of interconnecting flow channels are enclosed within a second enclosure that is housed inside a first enclosure. ### Claim 7 (depends on 6) The bioreactor of claim 6, wherein the fresh media is fed to the first cascade by a one or more reservoir and the cell culture is fed to the second cascade by a one or more reservoir. ### Claim 8 (depends on 7) The bioreactor as claimed in claim 7, wherein the second enclosure is supplied with a fresh gas facilitating a gas exchange for the cell culture during cell cultivation and to maintain a constant volume of the fresh gas which is increased intermittently. ### Claim 9 (depends on 8) The bioreactor as claimed in claim 8, wherein the first enclosure is infused with a one or more sterilizing means to eliminate cross contamination during the cell cultivation. ### Claim 10 (depends on 1) The bioreactor as claimed in claim 1 wherein the first and the second set of troughs comprises a set of cell bags each having a nadir at the bottom, that are partly filled with cell culture wherein the cell culture at the bottom is removed through the nadirs, in that, the set of troughs comprising at least one trough and the set of cell bags comprising at least one cell bag. ### Claim 11 A bioreactor for cell culture comprising at least one trough participating to form a cascade, the cascade housed within a first enclosure containing a fresh gas, the cascade operative to circulate a cell culture to interact with the fresh gas, wherein said at least one trough comprising an overflow outlet at the top to circulate said cell culture through said cascade and an outlet at the bottom coupled to a valve for collecting grown cell culture that settle down due to gravity. ### Claim 12 (depends on 11) The bioreactor of claim 11, further comprising a set of troughs participating to form the cascade wherein each trough in the set of trough having the overflow outlet at the top to circulate said cell culture through said cascade. ### Claim 13 (canceled) ### Claim 14 (depends on 12) The bioreactor of claim 12, further comprising a set of flow channels coupled between the overflow outlet of one trough to an inlet of another trough that is next in the set of troughs to form the cascade. ### Claim 15 (depends on 14) The bioreactor of claim 14, wherein the first enclosure is housed within a second enclosure and the space between the first enclosure and the second enclosure is infused with a sterile medium to eliminate cross contamination during the cell cultivation. ### Claim 16 A method of cultivation of cell culture in a bioreactor comprising: cascading a fresh medium through a first set of troughs; cascading a cell culture through a second set of troughs; allowing a grown cell culture to settle down due to gravity at the bottom of each of the second set of troughs, while cascading a remaining cell culture through the second set of troughs; and collecting a first quantity of the grown cell culture from the bottom of the second set of troughs; ### Claim 17 (depends on 16) The method of claim 16, further comprising replenishing a second quantity of the fresh media from the first set of troughs to the second set of trough, in that the first quantity of the grown cell culture is proportional to the second quantity of the fresh media. ### Claim 18 (depends on 17) The method of claim 17, wherein the collecting the first quantity of the grown cell culture and the replenishing of second quantity of the fresh media is continuous, in that, the second quantity of the fresh media is substantially lesser than the remaining cell culture in the bioreactor. ### Claim 19 (depends on 17) The method of claim 17, wherein the collecting the first quantity of the grown cell culture and the receiving of the second quantity replenishing fresh media into the bioreactor is intermittent. ### Claim 20 (depends on 16) The method of claim 16, further comprising charging the first cascade with the fresh medium and charging the second cascade with a fresh cell culture when the grown cell culture is collected. ### Claim 21 (depends on 20) The method of claim 20, further comprising charging the second cascade in part with the grown cell culture collected. ### Claim 22 (depends on 16) The method of claim 16, wherein the cascading comprises at least one of allowing a free flow of the fresh medium and the cell culture from one trough to another, rocking the first and second set of troughs and guiding through channels the fresh medium and the cell culture from one trough to another. ### Claim 23 (depends on 16) The method of claim 16, further comprising maintaining a fresh gas at a first temperature and maintaining the cell culture at a second temperature within the bioreactor. ### Claim 24 A method of growing a cell culture comprising cascading the cell culture through at least one trough housed within a first enclosure containing a fresh gas, the cascading enabling the cell culture to interact with the fresh gas. ### Claim 25 (depends on 24) The method of claim 24, further comprising cascading the cell culture through a set of troughs that are open at the top. ### Claim 26 (depends on 25) The method of claim 25, further comprising collecting grown cell culture that settle down in each trough in the set of troughs through a set of outlets at the bottom and a set of valves coupled to the set of outlets. ### Claim 27 (depends on 26) The method of claim 26, further comprising cascading the cell culture through a set of flow channels coupled between a set of overflow outlets in the set of troughs to a set of inlets in the next of the set of troughs. ### Claim 28 (depends on 28) The method of claim 28, further comprising preventing cross contamination of cell culture by infusing a sterile medium into a space between the first enclosure and a second enclosure, wherein the first enclosure is within the second enclosure.