# US 2022/0033751 A1 · Modular Bioreactor

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- Number: US 2022/0033751 A1
- Document ID: US20220033751A1
- Status: Published application
- Kind: A1
- Application number: 17/276,245
- Filed: 2019-10-03
- Published: 2022-02-03
- Earliest claimed: 2018-10-03
- Assignees: Pluristem Ltd.
- Applicants: Pluristem Ltd.
- Inventors: Lior RAVIV; Nadav ESHKOL; Dorina ROBERMAN
- Web page: https://patentgrep.com/patent/US20220033751A1

## CPC

- C12M 23/58: {Reaction vessels connected in series or in parallel (combinations of bioreactors with other apparatus, C12M43/00)}
- C12M 23/44: {Multiple separable units; Modules}
- C12M 25/14: {Scaffolds; Matrices (in general C12N5/0068)}
- C12M 29/04: {Filters; Permeable or porous membranes or plates, e.g. dialysis}
- C12M 29/10: {Perfusion}
- C12M 41/26: {of pH}
- C12M 41/32: {of substances in solution}
- C12M 41/34: {of gas}
- C12M 41/48: {Automatic or computerized control (automatic analysis G01N35/00)}
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}

## Abstract

The present disclosure relates to use of systems for culturing, incubating, and/or expanding adherent cells.

## Claims

### Claim 1

A cell culture apparatus, comprising: (a) a central medium container, said central medium container comprising a cell culture medium, wherein said central medium container does not contain cells; and (b) a plurality of 3-D culture vessels, wherein each of said 3-D culture vessels comprises cell carriers, comprising a 3-D matrix; wherein said central medium container is operably connected to said culture vessels via medium conduits that direct said medium from said central medium container through said 3-D matrix of said culture vessels in a parallel configuration.

### Claim 2 (depends on 1)

The cell culture apparatus of claim 1, wherein said a 3-D matrix is a fibrous matrix.

### Claim 3 (depends on 2)

The cell culture apparatus of claim 2, wherein said fibrous matrix is a synthetic matrix.

### Claim 4 (depends on 3)

The cell culture apparatus of claim 3, wherein said culture vessels comprise living cells.

### Claim 5 (depends on 4)

The method of claim 4, wherein said culture vessels are further operably connected to a means of measuring viability of said living cells.

### Claim 6 (depends on 1)

The cell culture apparatus of claim 1, wherein said central medium container comprises a means of monitoring and controlling pH of said medium.

### Claim 7 (depends on 1)

The cell culture apparatus of claim 1, wherein said central medium container comprises a means of monitoring and controlling dissolved oxygen concentration.

### Claim 8 (depends on 1)

The cell culture apparatus of claim 1, wherein said central medium container comprises a means of collecting data on conditions within said central medium container.

### Claim 9 (depends on 1)

The cell culture apparatus of claim 1, wherein said cell culture apparatus comprises a means of collecting data on transfer of fluid into and/or out of each of said culture vessels.

### Claim 10 (depends on 1)

The cell culture apparatus of claim 1, wherein said cell culture apparatus comprises a means of controlling a flow rate of fluid material transferred into each of said culture vessels.

### Claim 11 (depends on 1)

The cell culture apparatus of claim 1, wherein said cell culture apparatus is aseptic.

### Claim 12 (depends on 4)

The cell culture apparatus of claim 4, wherein said cells are adherent stromal cells.

### Claim 13 (depends on 12)

The cell culture apparatus of claim 12, wherein said adherent stromal cells are placenta-derived.

### Claim 14 (depends on 12)

The cell culture apparatus of claim 12, wherein said adherent stromal cells are derived from adipose tissue or bone marrow.