# US 2022/0033749 A1 · Cell culture chip > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2022/0033749 A1 - Document ID: US20220033749A1 - Status: Published application - Kind: A1 - Application number: 17/275,789 - Filed: 2019-09-11 - Published: 2022-02-03 - Earliest claimed: 2018-09-28 - Assignees: Ushio Denki Kabushiki Kaisha - Applicants: Ushio Denki Kabushiki Kaisha - Inventors: Makoto Yamanaka - Web page: https://patentgrep.com/patent/US20220033749A1 ## CPC - B01L 3/502: {with fluid transport, e.g. in multi-compartment structures} - C12M 23/16: {Microfluidic devices; Capillary tubes (integrated microfluidic structures B01L3/5027; microreactors B01J19/0093)} - C12M 23/12: {Well or multiwell plates (C12M25/04 takes precedence)} - C12M 23/20: {Material Coatings (immunocoatings C12M25/00)} - B01L 2300/0829: Multi-well plates; Microtitration plates - B01L 2300/0851: Bottom walls - B01L 2400/0406: capillary forces - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS} ## Abstract A cell culture chip from which a liquid retained in an opening is able to be sucked by a simple operation procedure while a liquid in a channel remains is provided. A cell culture chip according to the present invention includes a bottom portion; a base portion formed on an upper surface of the bottom portion; a first well provided by opening the base portion in a first direction extending from a portion of a main surface of the base portion toward the bottom portion and having a shape such that a capillary force thereof is smaller than that of the chamber; a second well provided by opening the base portion in the first direction at a position separated from the first well in a second direction parallel to the main surface; and a tubular chamber that is defined by a region sandwiched between the bottom portion and the base portion and that provides communication between the first and second wells in the second direction. The first well has a shape with which a capillary force of the first well is smaller than a capillary force of the chamber. ## Claims ### Claim 1 A cell culture chip comprising: a bottom portion; a base portion formed on an upper surface of the bottom portion; a first well provided by opening the base portion in a first direction extending from a portion of a main surface of the base portion toward the bottom portion, the main surface being a surface opposite to the bottom portion; a second well provided by opening the base portion in the first direction at a position separated from the first well in a second direction parallel to the main surface; and a tubular chamber that is defined by a region sandwiched between the bottom portion and the base portion and that provides communication between the first well and the second well in the second direction, wherein the first well has a shape such that a capillary force of the first well is smaller than a capillary force of the chamber. ### Claim 2 (depends on 1) The cell culture chip according to claim 1, wherein the first well has a reduced-diameter region of which an opening diameter continuously decreases without increasing toward the bottom portion at a position closer to the bottom portion than the main surface of the base portion. ### Claim 3 (depends on 2) The cell culture chip according to claim 2, wherein the first well has an inner wall defined by the base portion, and wherein the inner wall includes a curved surface or a flat surface non-parallel to the main surface in the reduced-diameter region of the first well. ### Claim 4 (depends on 2) The cell culture chip according to claim 2, comprising: a communication well that is formed continuously with the first well in the first direction and that provides communication between the reduced-diameter region of the first well and the chamber in the first direction, wherein the communication well has a bottom surface defined by the bottom portion and has an opening diameter smaller than the opening diameter of the reduced-diameter region of the first well. ### Claim 5 (depends on 3) The cell culture chip according to claim 3, wherein a bottom surface of the first well is defined by the bottom portion. ### Claim 6 (depends on 1) The cell culture chip according to claim 1, wherein the second well has a shape such that a capillary force of the second well is smaller than a capillary force of the chamber. ### Claim 7 (depends on 6) The cell culture chip according to claim 6, wherein the second well has a reduced-diameter region of which an opening diameter continuously decreases without increasing toward the bottom portion at a position closer to the bottom portion than the main surface of the base portion. ### Claim 8 A cell culture chip comprising: a bottom portion; a base portion formed on an upper surface of the bottom portion; a first well provided by opening the base portion in a first direction extending from a portion of a main surface of the base portion toward the bottom portion, the main surface being a surface opposite to the bottom portion; a second well provided by opening the base portion in the first direction at a position separated from the first well in a second direction parallel to the main surface; and a tubular chamber that is defined by a region sandwiched between the bottom portion and the base portion and that provides communication between the first well and the second well in the second direction, wherein the first well has a reduced-diameter region of which an opening diameter continuously decreases without increasing at a position closer to the bottom portion than the main surface of the base portion. ### Claim 9 (depends on 8) The cell culture chip according to claim 8, wherein the first well has an inner wall defined by the base portion, and wherein the inner wall includes a curved surface or a flat surface non-parallel to the main surface in the reduced-diameter region of the first well. ### Claim 10 (depends on 9) The cell culture chip according to claim 9, wherein the second well has a reduced-diameter region in which an opening diameter continuously decreases without increasing at a position closer to the bottom portion than the main surface of the base portion. ### Claim 11 (depends on 3) The cell culture chip according to claim 3, comprising: a communication well that is formed continuously with the first well in the first direction and that provides communication between the reduced-diameter region of the first well and the chamber in the first direction, wherein the communication well has a bottom surface defined by the bottom portion and has an opening diameter smaller than the opening diameter of the reduced-diameter region of the first well. ### Claim 12 (depends on 2) The cell culture chip according to claim 2, wherein a bottom surface of the first well is defined by the bottom portion. ### Claim 13 (depends on 8) The cell culture chip according to claim 8, wherein the second well has a reduced-diameter region in which an opening diameter continuously decreases without increasing at a position closer to the bottom portion than the main surface of the base portion.