# US 2022/0033737 A1 · Compositions for medical instrument cleaning > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2022/0033737 A1 - Document ID: US20220033737A1 - Status: Published application - Kind: A1 - Application number: 17/276,303 - Filed: 2019-09-17 - Published: 2022-02-03 - Earliest claimed: 2018-09-27 - Assignees: Danisco US Inc. - Applicants: Danisco US Inc - Inventors: Arjen HOEKSTRA; Michael STONER; Zhenfeng ZHAO - Web page: https://patentgrep.com/patent/US20220033737A1 ## CPC - C11D 3/38618: {Protease or amylase in liquid compositions only} - C11D 3/48: Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions - C11D 1/72: Ethers of polyoxyalkylene glycols - C11D 3/38627: {containing lipase} - C11D 3/38663: {Stabilised liquid enzyme compositions} - C11D 3/43: Solvents - C11D 2111/14: Hard surfaces - C11D: DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL ## Abstract Disclosed herein are compositions comprising a nonionic surfactant and one or more inherently stable subtilisin, and methods related to the use of such compositions for the cleaning of medical and dental instruments. ## Claims ### Claim 1 A medical or dental instrument detergent composition comprising: between about 1% to 15% by weight of a nonionic surfactant; between about 250 ppm and about 10000 ppm of an inherently stable subtilisin variant wherein the composition does not comprise a substantial amount of a protease stabilizer. ### Claim 2 (depends on 1) The detergent composition of claim 1, wherein the composition comprises less than about 0.01% by weight, of a protease stabilizer. ### Claim 3 (depends on 2) The detergent composition of claim 2, wherein the composition comprises less than about 0.001% by weight of a protease stabilizer. ### Claim 4 (depends on 1) The detergent composition of claim 1, wherein the composition does not comprise a protease stabilizer selected from the group consisting of a protease inhibitor, peptide aldehyde, an organoboron compound, or boronic acid derivative. ### Claim 5 (depends on 4) The detergent composition of claim 4, wherein the boronic acid derivative is phenyl boronic acid (PBA) or 4-formylphenyl-boronic acid (FPBA). ### Claim 6 (depends on 1) The detergent composition of claim 1, wherein the nonionic surfactant is a C6 to C20 alcohol ethoxylate with 2 to 14 moles of ethoxylation. ### Claim 7 (depends on 6) The detergent composition of claim 6, wherein the nonionic surfactant is selected from the group of polyoxyalkylene alkyl ethers, polyalkylene glycols, alkylamine oxides, polyoxyalkylene, alkyl phenyl ethers, fatty acid polyoxyethylene esters, fatty acid sorbitan esters, fatty acids polyoxyalkylene sorbitan esters, fatty acid saccharide esters, alkyl polysaccharides, alkyl glyceryl ethers, and fatty acid alkanolamides. ### Claim 8 (depends on 1) The detergent composition of claim 1, wherein the composition further comprises between about 10-30% by weight of at least one organic solvent. ### Claim 9 (depends on 8) The detergent composition of claim 8, wherein the solvent is selected from the group consisting of polyols such as glycerol, propane-1,2-diol or propane-1,3-diol. ### Claim 10 (depends on 1) The detergent composition of claim 1, wherein the composition further comprises, from about 10% to 30% by weight of a biodegradable chelating agent. ### Claim 11 (depends on 11) The detergent composition of claim 11, wherein the biodegradable chelating agent is selected from the group of sodium salts of glutamic acid diacetic acid (GLDA), methylglycinediacetic acid (MGDA), and itaconic acid. ### Claim 12 A method for cleaning a medical or dental instrument comprising: a) contacting the medical or dental instrument in a detergent for medical or dental instrument cleaning comprising between about 1% to 15% by weight of a nonionic surfactant; between about 250 ppm and about 10000 ppm of an inherently stable subtilisin variant; wherein the composition does not comprise a substantial amount of a protease stabilizer; b) allowing the instrument to be contacted for a period of time sufficient to reduce or remove soils on the instrument; and c) optionally rinsing the instrument. ### Claim 13 (depends on 12) The method of claim 12, wherein the instrument is contacted with the detergent for at least 1 minute. ### Claim 14 (depends on 13) The method of claim 13, wherein the instrument is contact with the detergent for an amount of time up to 24 hours. ### Claim 15 (depends on 12) The method of claim 12, wherein the instrument is contacted with the detergent for between a time of 1-60 minutes? ### Claim 16 (depends on 12) The method of claim 12, wherein the instrument is contacted with the detergent at a temperature between 30 degrees and 70 degrees Celsius. ### Claim 17 (depends on 16) The method of claim 16, wherein the instrument is contacted with the detergent at a temperature between 40 degrees and 60 degrees Celsius. ### Claim 18 (depends on 12) The method of claim 12, wherein the composition comprises less than about 0.01% by weight, of a protease stabilizer. ### Claim 19 (depends on 18) The method of claim 18, wherein the composition comprises less than about 0.001% by weight, of a protease stabilizer. ### Claim 20 (depends on 12) The method of claim 12, wherein the composition does not comprise a protease stabilizer selected from the group consisting of a peptide aldehyde, organoboronic acid, or boronic derivative. ### Claim 21 (depends on 20) The method of claim 20, wherein the boronic acid derivative is phenyl boronic acid (PBA) or 4-formylphenyl-boronic acid (4-FPBA). ### Claim 22 (depends on 12) The method of claim 12, wherein the nonionic surfactant is a C6 to C20 alcohol ethoxylate with 2 to 14 moles of ethoxylation. ### Claim 23 (depends on 22) The method of claim 22, wherein the nonionic surfactant is selected from the group of polyoxyalkylene alkyl ethers, polyalkylene glycols, alkylamine oxides, polyoxyalkylene alkyl phenyl ethers, fatty acid polyoxyethylene esters, fatty acid sorbitan esters, fatty acids polyoxyalkylene sorbitan esters, fatty acid saccharide esters, alkyl polysaccharides, alkyl glyceryl ethers, and fatty acid alkanolamides. an alcohol ethoxylate. ### Claim 24 (depends on 12) The method of claim 12, wherein the composition further comprises between about 10-30% by weight of at least one organic solvent. ### Claim 25 (depends on 24) The method of claim 24, wherein the solvent is selected from the group consisting of propylene glycol, glycerol, propane-1,2-diol or propane-1,3-diol. ### Claim 26 (depends on 12) The method of claim 12, wherein the composition further comprises, from about 10% to 30% by weight of a biodegradable chelating agent. ### Claim 27 (depends on 26) The detergent composition of claim 26, wherein the biodegradable chelating agent is selected from the group of sodium salts of glutamic acid diacetic acid (GLDA), methylglycinediacetic acid (MGDA), and itaconic acid.