# US 2022/0033451 A1 · Self-assembly of protein-based biomaterials with multiple morphologies

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- Number: US 2022/0033451 A1
- Document ID: US20220033451A1
- Status: Published application
- Kind: A1
- Application number: 17/343,380
- Filed: 2021-06-09
- Published: 2022-02-03
- Earliest claimed: 2008-11-14
- Assignees: Bondwell Technologies LP
- Applicants: Bondwell Technologies LP
- Inventors: Sarah E. Bondos; Alexandra M. Whiteley; Kathleen S. Matthews; Zhao Huang; Autumn Brawley; Jan Patterson
- Web page: https://patentgrep.com/patent/US20220033451A1

## CPC

- C07K 14/4702: {Regulators; Modulating activity}
- A61P 17/02: for treating wounds, ulcers, burns, scars, keloids, or the like
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The present disclosure relates, in some embodiments, to a composition comprising a biomaterial. A biomaterial may comprise, for example, one or more molecules capable of self-association and/or self-assembly. In some embodiments, a biomaterial may comprise one or more polypeptides and/or proteins. A biomaterial may comprise, for example, two or more self-assembled Ultrabithorax (Ubx) protein molecules. A Ubx protein, in some embodiments, may be any wild type Drosophila melanogaster Ultrabithorax protein, including any natural or non-natural isoforms (e.g., alternative splicing isoforms). The present disclosure relates, in some embodiments, to a method of making a biomaterial comprising contacting two or more Ubx protein molecules under conditions that permit self-assembly to form a first fibril and contacting the first fibril to a second fibril.

## Claims

### Claim at position 1

1-12. (canceled)

### Claim 13

A method of making a biomaterial comprising a Ubx protein, the method comprising: a) concentrating a Ubx protein solution, b) exposing the Ubx protein solution to a metal chelator to self-assemble Ubx proteins and c) recovering the recovering assembled Ubx material(s) proteins.

### Claim 14 (depends on 13)

A method according to claim 13 wherein the metal chelator is selected from ethylenediaminetetraacetic acid (EDTA), (ethylenebis(oxyethylenenitrilo))tetraacetic acid (EGTA), and 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid (BAPTA).

### Claim 15 (depends on 13)

A method according to claim 13 wherein step b) further comprises waiting for at least 2 hours after exposing the Ubx protein solution to a metal chelator.

### Claim 16 (depends on 13)

A method according to claim 13 wherein the biomaterial comprising Ubx is a fibril, film, fiber, sheet, bundle, lattice, or encapsulate.

### Claim 17

A method of using a biomaterial comprising Ubx, the method comprising: a) preparing a structure comprising Ubx and having a morphology selected from fibril, film, fiber, sheet, bundle, lattice, or encapsulate, and b) administering the structure to an animal or human in need thereof.

### Claim 18 (depends on 17)

A method according to claim 17, wherein the structure is a lattice and the method further comprises seeding the lattice with cells to engineer tissues.

### Claim 19 (depends on 17)

A method according to claim 17, wherein the structure is a film and the method further comprises coating a surface of a biological implant with the film.

### Claim 20 (depends on 17)

A method according to claim 17, wherein the structure is a fiber and the method further comprises stacking fibers to create porous three dimensional structures and used for filtration.

### Claim 21 (depends on 17)

A method according to claim 17, wherein the structure is a bundle, and the method further comprises using the bundle as a medical suture.

### Claim 22

A method of preparing a product from one or more reactants, the method comprising: a) providing a serial fiber having a first domain comprising a first chimera comprising a first Ubx protein and a first catalyst and a second domain comprising a second chimera comprising a second Ubx protein and a second catalyst, wherein the first catalyst is operable to react with at least one reactant to form at least one intermediate and the second catalyst is operable to react with the at least one intermediate to produce the product; b) contacting a composition comprising the one or more reactants with the first domain of the serial fiber under conditions that permit formation of the at least one intermediate; and c) flowing the composition along the fiber to contact the second domain under conditions that permit formation of the product.

### Claim 23 (depends on 22)

A method according to claim 22 wherein the first catalyst is a first enzyme, the second catalyst is a second enzyme, or the first catalyst is a first enzyme and the second catalyst is a second enzyme.

### Claim 24 (depends on 22)

A method according to claim 22 wherein the first Ubx protein is the same as the second Ubx protein.