# US 2022/0032256 A1 · Devices and methods for light-directed polymer synthesis

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- Number: US 2022/0032256 A1
- Document ID: US20220032256A1
- Status: Published application
- Kind: A1
- Application number: 17/368,697
- Filed: 2021-07-06
- Published: 2022-02-03
- Earliest claimed: 2020-07-07
- Assignees: Twist Bioscience Corporation
- Applicants: Twist Bioscience Corporation
- Inventors: Jeremy LACKEY; Scott INDERMUEHLE; David DODD; Stefan PITSCH
- Web page: https://patentgrep.com/patent/US20220032256A1

## CPC

- B01J 19/0046: {Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays (synthesis methods per se C40B50/00)}
- C07H 1/00: Processes for the preparation of sugar derivatives
- C07H 21/04: with deoxyribosyl as saccharide radical
- C08G 61/00: Macromolecular compounds obtained by reactions forming a carbon-to-carbon link in the main chain of the macromolecule (C08G2/00 - C08G16/00 take precedence)
- C08G 61/12: Macromolecular compounds containing atoms other than carbon in the main chain of the macromolecule
- C08L 65/00: Compositions of macromolecular compounds obtained by reactions forming a carbon-to-carbon link in the main chain (C08L7/00 - C08L57/00, C08L61/00 take precedence); Compositions of derivatives of such polymers
- B01J 2219/00317: Microwell devices, i.e. having large numbers of wells
- B01J 2219/00596: Solid-phase processes
- B01J 2219/00711: Light-directed synthesis
- B01J 2219/00722: Nucleotides
- H10W 90/00: Package configurations
- B01J: CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- C07H: SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS (derivatives of aldonic or saccharic acids C07C, C07D; aldonic acids, saccharic acids C07C59/105, C07C59/285; cyanohydrins C07C255/16; glycals C07D; compounds of unknown constitution C07G; polysaccharides, derivatives thereof C08B; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; sugar industry C13)
- C08G: MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08L: COMPOSITIONS OF MACROMOLECULAR COMPOUNDS (compositions based on polymerisable monomers C08F, C08G; artificial filaments or fibres D01F; textile treating compositions D06)
- H10W: GENERIC PACKAGES, INTERCONNECTIONS, CONNECTORS OR OTHER CONSTRUCTIONAL DETAILS OF DEVICES COVERED BY CLASS H10

## Abstract

Provided herein are compositions, devices, systems and methods for generation and use of biomolecule-based information for storage. Further provided are devices comprising addressable LED arrays to control polynucleotide synthesis (deprotection, extension, or cleavage, etc.) The compositions, devices, systems and methods described herein provide improved storage, density, and retrieval of biomolecule-based information.

## Claims

### Claim 1

A device for polymer synthesis, comprising:
a solid support, wherein the solid support comprises a plurality of wells, wherein each of the wells comprises:
a) a synthesis surface located in a bottom region of each of the wells;
b) a light-emitting layer in addressable communication with the synthesis surface and situated below the synthesis surface; and
c) a CMOS driver located in addressable communication with the light-emitting layer.

### Claim 2 (depends on 1)

The device of claim 1, wherein the light-emitting layer is a light-emitting diode (LED).

### Claim 3 (depends on 2)

The device of claim 2, wherein the LED is an organic LED (OLED) or a micro-LED.

### Claim 4

(canceled)

### Claim 5 (depends on 1)

The device of claim 1, wherein the light-emitting layer emits ultraviolet (UV) light visible light, or infrared (IR) light.

### Claim 6 (depends on 5)

The device of claim 5, wherein the UV light has a wavelength of about 350, 365, or 400 nm.

### Claim at position 7

7-9. (canceled)

### Claim 10 (depends on 5)

The device of claim 5, wherein the visible light has a wavelength of about 405 or 450 nm.

### Claim 11 (depends on 5)

The device of claim 5, wherein the visible light has a wavelength of about 450 nm.

### Claim 12

(canceled)

### Claim 13 (depends on 5)

The device of claim 5, wherein the IR light has a wavelength of about 800 nm.

### Claim 14 (depends on 1)

The device of claim 1, wherein the solid support comprises addressable loci at a density of at least 10×106 addressable loci per cm2.

### Claim 15

(canceled)

### Claim 16 (depends on 1)

The device of claim 1, wherein the solid support comprises addressable loci, and each addressable locus comprises a diameter up to about 1000 nm.

### Claim 17

(canceled)

### Claim 18 (depends on 1)

The device of claim 1, wherein each of the wells comprises a depth of 100 nm to 1000 nm.

### Claim at position 17

19-22. (canceled)

### Claim 23

A method for synthesizing a polymer, comprising:
a) providing a solid support comprising a surface;
b) depositing at least one nucleoside on the surface, wherein the at least one nucleoside couples to a polynucleotide attached to the surface, wherein the coupling comprises a light-directed deprotection step by a light-emitting layer, and wherein the light-emitting layer is located beneath the surface; and
c) repeating step b) to synthesize a plurality of polynucleotides on the surface, wherein polynucleotides having different sequences on the surface are present at a density of at least 100×106 polynucleotides per cm2.

### Claim 24 (depends on 23)

The method of claim 23, wherein the light-emitting layer is a light-emitting diode (LED).

### Claim 25 (depends on 24)

The method of claim 24, wherein the LED is an organic LED (OLED) or a micro-LED.

### Claim 26

(canceled)

### Claim 27 (depends on 23)

The method of claim 23, wherein the light-emitting layer emits ultraviolet (UV) light, visible light, or infrared (IR) light.

### Claim 28 (depends on 27)

The method of claim 27, wherein the light has a wavelength of about 350, 365, 400, 405, 450, or 800 nm.

### Claim at position 24

29-38. (canceled)

### Claim 39 (depends on 23)

The method of claim 23, wherein the deprotection step deprotects a 5′-hydroxyl group.

### Claim 40 (depends on 39)

The method of claim 39, wherein the 5′-hydroxyl group is protected by a protecting group of the formula:
wherein each R, R1, and R2 is independently is selected from a group consisting of: —C(O)R3, —C(O)OR3, —C(O)NR3R4, —SOR3, —SO2R4, alkyl, alkenyl, alkynyl, aryl, heteroaryl, or heterocyclyl, each of which is independently substituted or unsubstituted, or hydrogen, or R1 and R2 together with the nitrogen atom to which R1 and R2 are bound form a ring, wherein the ring is substituted or unsubstituted; wherein each R3 and R4 is independently —C(O)R5, —C(O)OR5, —C(O)NR5R6, —OR5, —SR5, —NR5R6, —NR5C(O)R6, —OC(O)R5, alkyl, alkenyl, alkynyl, aryl, heteroaryl, or heterocyclyl, each of which is independently substituted or unsubstituted, or hydrogen or halogen; wherein each R5 and R6 is independently alkyl, alkenyl, alkynyl, aryl, heteroaryl, or heterocyclyl, each of which is independently substituted or unsubstituted, or hydrogen.

### Claim 41 (depends on 39)

The method of claim 39, wherein the 5′-hydroxyl group is protected by a nitrophenylpropyloxycarbonyl (NPPOC) protecting group.

### Claim 42 (depends on 39)

The method of claim 39, wherein the 5′-hydroxyl group is protected by a 2,(3,4-methylenediooxy-6-nitrophenyl)propoxycarbonyl (MNPPOC) group.