# US 2022/0030896 A2 · Choline ester-containing composition for oral ingestion > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2022/0030896 A2 - Document ID: US20220030896A2 - Status: Published application - Kind: A2 - Application number: 16/341,748 - Filed: 2017-10-16 - Published: 2022-02-03 - Earliest claimed: 2016-10-14 - Assignees: Shinshu University - Applicants: Shinshu University - Inventors: KOZO NAKAMURA - Web page: https://patentgrep.com/patent/US20220030896A2 ## CPC - A23L 33/105: Plant extracts, their artificial duplicates or their derivatives - A61K 9/08: Solutions {(composition of solutions A61K47/00)} - A61K 9/19: lyophilised {, i.e. freeze-dried, solutions or dispersions (lyophilised products with subsequent particle size reduction A61K9/14; granules or pellets made by lyphilisation A61K9/1682; solid oral dosage forms made by lyophilisation A61K9/2095; lyophilisation additives A61K47/00)} - A61K 31/221: with compounds having an amino group, e.g. acetylcholine, acetylcarnitine - A61K 36/81: Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed - A61K 36/899: Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane - A61K 47/12: Carboxylic acids; Salts or anhydrides thereof - A61P 9/12: Antihypertensives - A23V 2002/00: Food compositions, function of food ingredients or processes for food or foodstuffs - A23L: FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF (preservation thereof A23B) - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS - A23V: INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION ## Abstract The object of the present invention is to provide a composition having a blood pressure-lowering effect and/or an anti-stress effect, wherein an active ingredient of a choline ester such as acetylcholine, and the like, can be easily administered to humans by the oral pathway. Consequently, the object of the present invention is to provide a novel composition for oral ingestion having a blood pressure-lowering effect and a vasodilatory effect, wherein a choline ester such as acetylcholine, and the like, can be easily administered to humans by the oral pathway, and to provide food which is a useful supply source therefore. ## Claims ### Claim 1 A composition having a blood pressure-lowering effect and/or an anti-stress effect and comprising a choline ester as an active ingredient, wherein the composition is for oral ingestion and wherein the choline ester content is 5 μg to 50 mg. ### Claim 2 (depends on 1) The composition according to claim 1, wherein the composition is a food composition. ### Claim 3 (depends on 1) The composition according to claim 1, wherein the composition is a pharmaceutical composition for lowering blood pressure and/or for anti-stress. ### Claim 4 (depends on 1, 2, 3) The composition according to any one of claims 1 to 3, wherein the choline ester is derived from an edible plant. ### Claim 5 (depends on 1, 2, 3, 4) The composition according to any one of claims 1 to 4, consisting of a lyophilized powder and/or a hot air-dried powder of edible plants. ### Claim 6 (depends on 5) The composition according to claim 5, consisting of a lyophilized powder and/or a hot air-dried powder of edible plants, which can pass through a 20 mesh sieve. ### Claim 7 (depends on 1, 2, 3, 4) The composition according to any one of claims 1 to 4, wherein the composition is an extract obtained by extracting edible plants with ethanol or hydrous ethanol. ### Claim 8 (depends on 4, 5, 6, 7) The composition according to any one of claims 4 to 7, wherein the edible plant is a fruit of a Solanaceae Solanum eggplant species (Solanum melongena) and/or a young shoot of a bamboo subfamily of Piceaceae (Poaceae, Bambusoideae, Bambuseae). ### Claim 9 (depends on 1, 2, 3) The composition according to any one of the claims 1 to 3, wherein the choline ester comprises one or more selected from the group consisting of acetylcholine, butyrylcholine and propionylcholine. ### Claim 10 (depends on 9) The composition according to claim 9, wherein the choline ester does not contain lactoylcholine. ### Claim 11 (depends on 1, 2, 3, 4, 5, 6, 7, 8, 9, 10) The composition according to any one of claims 1 to 10, wherein the concentration of choline ester is 5 μg/g to 250 mg/g and the intake amount per day is adjusted from 5 μg to 50 mg. ### Claim 12 (depends on 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11) The composition according to any one of claims 1 to 11, wherein the composition is frozen. ### Claim 13 (depends on 1, 2, 3) The composition according to any one of claims 1 to 3, wherein the composition is a part or the whole of the frozen fruit of a Solanaceae Solanum eggplant species (Solanum melongena). ### Claim 14 A method of producing a composition for oral ingestion having a blood pressure-lowering effect and/or an anti-stress effect and comprising a choline ester as an active ingredient, wherein the method comprises a step for making an edible plant into a lyophilized powder and/or a hot air-dried powder, and a step for dispensing the lyophilized powder and/or the hot air-dried powder so that the choline ester content is 5 μg to 50 mg. ### Claim 15 (depends on 14) The method according to claim 14, wherein the edible plant is a fruit of a Solanaceae Solanum eggplant species (Solanum melongena) and/or a young shoot of a bamboo subfamily of Piceaceae (Poaceae, Bambusoideae, Bambuseae). ### Claim 16 (depends on 14, 15) The method according to claim 14 or 15, wherein the method further comprises a step for heating the edible plant. ### Claim 17 (depends on 14, 15, 16) The method according to any one of claims 14 to 16, wherein the method further comprises a step for suspending the lyophilized powder and/or a hot air-dried powder of an edible plant in water, and a step for adding an acid to the suspension obtained. ### Claim 18 (depends on 17) The method according to claim 17, wherein the method further comprises a step for adjusting the suspension to which acid has been added to a pH of 5.5 to 4.5. ### Claim 19 (depends on 14, 15, 16, 17, 18) A composition for oral ingestion having a blood pressure-lowering effect and/or an anti-stress effect and comprising a choline ester as an active ingredient, which is produced by a method according to any one of claims 14 to 18. ### Claim 20 A method for producing an extract for oral ingestion having a blood pressure-lowering effect and/or an anti-stress effect and comprising a choline ester as an active ingredient, wherein the method comprises a step for extracting an edible plant or a lyophilized powder and/or a hot air-dried powder of an edible plant with ethanol or hydrous ethanol. ### Claim 21 (depends on 20) The method according to claim 20, wherein the method comprises a step for extracting an edible plant or a lyophilized powder and/or hot air-dried powder of an edible plant with ethanol. ### Claim 22 (depends on 20) The method according to claim 20, wherein the method comprises a step for extracting an edible plant or a lyophilized powder and/or a hot air-dried powder of an edible plant with ethanol, and wherein the ethanol concentration of the hydrous ethanol is 25 to 60% (w/w), or 95% (w/w) or more. ### Claim 23 (depends on 20, 21, 22) The method according to any one of claims 20 to 22, wherein L-ascorbic acid is added to the ethanol or the hydrous ethanol used for the extraction. ### Claim 24 (depends on 20, 21, 22, 23) The method according to any one claims 20 to 23, wherein the method comprises a step for adjusting the choline ester content of the extract from 5 μg to 50 mg. ### Claim 25 (depends on 20, 21, 22, 23, 24) An extract for oral ingestion having a blood pressure-lowering effect and/or an anti-stress effect and comprising a choline ester as an active ingredient produced according to any one of claims 20 to 24.