# US 2022/0030795 A1 · Lettuce variety nun 09178 ltl

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- Number: US 2022/0030795 A1
- Document ID: US20220030795A1
- Status: Published application
- Kind: A1
- Application number: 17/390,596
- Filed: 2021-07-30
- Published: 2022-02-03
- Earliest claimed: 2020-07-30
- Assignees: Nunhems B.V.
- Applicants: Nunhems B.V.
- Inventors: Johan VAN ZEE
- Web page: https://patentgrep.com/patent/US20220030795A1

## CPC

- A01H 6/1472: {Lactuca sativa [lettuce]}
- A01H 5/10: Seeds
- A01H 5/12: Leaves
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

A new and distinct lettuce variety NUN 09178 LTL is disclosed as well as seeds and plants and heads or leaves thereof. NUN 09178 LTL is a multileaf red lettuce variety, comprising resistance to Downy Mildew (Bremia lactucae) Isolates Bl:16-37 EU and Nasonovia ribisnigri Biotype Nr:0.

## Claims

### Claim 1

A plant, plant part, or seed of lettuce variety NUN 09178 LTL, wherein a representative sample of seed of said variety has been deposited under Accession Number NCIMB 43807.

### Claim 2 (depends on 1)

The plant part of claim 1, wherein the plant part is a head, a leaf, pollen, an ovule, a fruit, a scion, a rootstock, a cutting, a flower, or a cell.

### Claim 3 (depends on 1)

A seed that produces the plant of claim 1.

### Claim 4 (depends on 1)

A seed grown on the plant of claim 1.

### Claim 5 (depends on 4)

A lettuce plant grown from the seed of claim 4.

### Claim 6 (depends on 1)

A lettuce plant or a part thereof having all the physiological and morphological characteristics of the plant of claim 1, when grown under the same environmental conditions.

### Claim 7 (depends on 1)

A tissue or cell culture of regenerable cells of the plant or plant part of claim 1.

### Claim 8 (depends on 7)

The tissue or cell culture according to claim 7, comprising cells or protoplasts derived from a plant part suitable for vegetative reproduction, wherein the plant part is an embryo, a meristem, a cotyledon, a hypocotyl, a pollen, a leaf, an anther, a root, a root tip, a pistil, a petiole, a flower, a fruit, a seed, a stem, or a stalk.

### Claim 9 (depends on 7)

A lettuce plant regenerated from the tissue or cell culture of claim 7, wherein the plant has all of the physiological and morphological characteristics of the plant of lettuce variety NUN 09178 LTL, when determined at 5% significance level for numerical characteristics and determined by type or degree for non-numerical characteristics, when grown under the same environmental conditions, and wherein a representative sample of seed of said lettuce variety has been deposited under Accession Number NCIMB 43807.

### Claim 10 (depends on 1)

A method of producing of the plant of claim 1, said method comprising vegetative propagation of at least a part of the plant of lettuce variety NUN 09178 LTL, wherein a representative sample of seed of said lettuce variety has been deposited under Accession Number NCIMB 43807.

### Claim 11 (depends on 10)

The method of claim 10, wherein said vegetative propagation comprises regenerating a whole plant from a part of lettuce variety NUN 09178 LTL, wherein a representative sample of seed of said lettuce variety has been deposited under Accession Number NCIMB 43807.

### Claim 12 (depends on 10)

The method of claim 10, wherein said part is a cutting, a cell culture, or a tissue culture.

### Claim 13 (depends on 1)

A plant vegetatively propagated from the plant of claim 1, or part thereof, wherein the vegetatively propagated plant, or part thereof have all of the physiological and morphological characteristics of the plant of lettuce variety NUN 09178 LTL, when determined at 5% significance level for numerical characteristics and determined by type or degree for non-numerical characteristics, when grown under the same environmental conditions, and wherein a representative sample of seed of said lettuce variety has been deposited under Accession Number NCIMB 43807.

### Claim 14 (depends on 1)

A method of producing a lettuce plant, said method comprising crossing the plant of claim 1 with itself or a second lettuce plant, selecting a progeny lettuce plant from said crossing, allowing the progeny lettuce plant to form seed, and optionally repeating the crossing and selecting one or more times to produce further progeny seed.

### Claim 15 (depends on 1)

A first generation progeny of the lettuce plant of claim 1, obtained by crossing the plant of lettuce variety NUN 09178 LTL with itself or another lettuce plant.

### Claim 16 (depends on 1)

A method of producing an F1 hybrid lettuce seed, said method comprising crossing a first parent lettuce plant with a second parent lettuce plant and harvesting the resultant hybrid lettuce seed, wherein at the first parent lettuce plant or second parent lettuce plant is the plant of claim 1.

### Claim 17 (depends on 16)

An F1 hybrid lettuce seed produced by the method of claim 16.

### Claim 18 (depends on 17)

An F1 hybrid lettuce plant or part thereof, produced by growing the seed of claim 17.

### Claim 19 (depends on 2)

A food, a feed, or a processed product comprising the plant part of claim 2.

### Claim 20 (depends on 1)

A container comprising the plant, plant part, or seed of claim 1.

### Claim 21 (depends on 1)

A method of introducing a desired trait into the plant of claim 1, said method comprises transforming the plant of lettuce variety NUN 09178 LTL with a transgene that confers the desired trait, wherein a representative sample of seed of said lettuce variety has been deposited under Accession Number NCIMB 43807, and wherein the desired trait is yield, storage properties, color, enhanced nutritional quality, male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, environmental stress tolerance, modified carbohydrate metabolism, or modified protein metabolism.

### Claim 22 (depends on 21)

A lettuce plant produced by the method of claim 21, wherein the transformed plant contains the desired trait and otherwise retains all of the morphological and physiological characteristics of the plant of lettuce variety NUN 09178 LTL.

### Claim 23 (depends on 1)

A method of introducing a single locus conversion into the plant of claim 1, comprising:
a. crossing the plant of claim 1 with a second lettuce plant comprising a desired single locus conversion to produce F1 progeny plants;
b. selecting F1 progeny plants that have the single locus conversion to produce selected F1 progeny plants;
c. crossing the selected F1 progeny plants with lettuce variety NUN 09178 LTL to produce backcross progeny plants;
d. selecting backcross progeny plants that have the single locus conversion and otherwise comprise all of the physiological and morphological characteristics of lettuce variety NUN 09178 LTL to produce selected backcross progeny plants; and
e. repeating steps (c) and (d) one or more times in succession to produce selected second or higher backcross progeny plants that comprise the single locus and otherwise comprise all of the physiological and morphological characteristics of the plant of lettuce variety NUN 09178 LTL, wherein a representative sample of seed of said lettuce variety has been deposited under Accession Number NCIMB 43807.

### Claim 24 (depends on 23)

The method of claim 23, wherein the single locus confers yield, storage properties, color, enhanced nutritional quality, male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, environmental stress tolerance, modified carbohydrate metabolism, or modified protein metabolism.

### Claim 25 (depends on 23)

A lettuce plant produced by the method of claim 23, wherein the plant further comprises the single locus conversion and otherwise has all of the morphological and physiological characteristics of the plant of lettuce variety NUN 09178 LTL.

### Claim 26

A method of producing a modified lettuce plant, said method comprising mutating a target gene in lettuce plant or plant part of lettuce variety NUN 09178 LTL, wherein a representative sample of seed of said lettuce variety has been deposited under Accession Number NCIMB 43807.

### Claim 27 (depends on 26)

The method of claim 26, wherein the target gene modifies a desired trait and wherein the desired trait is yield, storage properties, color, enhanced nutritional quality, male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, environmental stress tolerance, modified carbohydrate metabolism, or modified protein metabolism or the mutation occurs in any of the following genes: Ferulate-5-hydrxylase, dmr1, dmr6, NCED4, PAL, PPO.

### Claim 28 (depends on 26)

The method of claim 26, wherein the target gene is mutated by targeted gene editing.

### Claim 29 (depends on 27)

A lettuce plant produced by the method of claim 27, wherein the modified lettuce plant contains the desired trait and otherwise has all of the physiological and morphological characteristics of lettuce variety NUN 09178 LTL.

### Claim 30 (depends on 1)

A method of producing doubled haploid cells of the plant of claim 1, said method comprising making doubled haploid cells from haploid cells from the plant or seed of lettuce variety NUN 09178 LTL, wherein a representative sample of seed of said lettuce variety has been deposited under Accession Number NCIMB 43807.

### Claim 31 (depends on 1)

A method for determining the genotype of the plant of claim 1, said method comprising obtaining a sample of nucleic acids from said plant and detecting in said nucleic acids a plurality of polymorphisms, thereby determining the genotype of the plant, and storing the results of detecting the plurality of polymorphisms on a computer readable medium.

### Claim 32 (depends on 1)

A method of producing a lettuce head or a lettuce leaf, said method comprising growing the plant of claim 1 until it develops at least a leaf or head, and collecting the leaf or head.

### Claim 33 (depends on 32)

A lettuce head or a lettuce leaf produced by the method of claim 32.

### Claim 34 (depends on 32)

A container comprising the lettuce head or lettuce leaf produced by the method of claim 32.

### Claim 35 (depends on 1)

A method of determining the genotype of the plant of claim 1, said method comprising obtaining a sample of nucleic acids from said plant and detecting in said nucleic acids a plurality of polymorphisms, thereby determining the genotype of the plant and storing the results of detecting the plurality of polymorphisms on a computer readable medium.

### Claim 36 (depends on 1)

A method for developing a lettuce plant in a lettuce breeding program, said method comprising applying plant breeding techniques comprising recurrent selection, backcrossing, pedigree breeding, mass selection, mutation breeding, genetic marker enhanced selection, or genetic transformation to the plant of claim 1 or part thereof, wherein said plant breeding techniques result in a development of a lettuce plant.

### Claim 37 (depends on 1)

A method of producing a lettuce plant derived from the plant of claim 1, comprising:
a. preparing a progeny lettuce plant derived from lettuce variety NUN 09178 LTL by crossing the plant of claim 1 with itself or with a second lettuce plant, wherein a representative sample of seed of said lettuce variety has been deposited under Accession Number NCIMB 43807;
b. crossing the progeny plant with itself or a second lettuce plant to produce seed of a progeny plant of a subsequent generation;
c. growing a progeny plant of a subsequent generation from said seed and crossing the progeny plant of a subsequent generation with itself or a second lettuce plant; and
d. repeating step (b) and/or (c) for at least one more generation to produce a lettuce plant derived from lettuce variety NUN 09178 LTL.