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US 2022/0028490 A1Published application
Methods for Analyzing Real Time Digital PCR Data
OwnerYan Wang, Cory McCluskeyinventors
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Abstract
Disclosed are methods for analyzing digital PCR data using real time measurements during the amplification cycles of the dPCR. An endpoint threshold is used to preliminarily separate positive amplifications from negative amplifications for a plurality of microreactions in the dPCR. The preliminary positive amplifications are further evaluated based on properties of the amplification curves of the microreactions so as to remove false positives.
Claims9
A computer-implemented method of analyzing dPCR data, comprising:
- a) collecting readings of microreactions of a plurality of microreactions during the dPCR amplification process;
- b) determining and displaying amplification curves for microreactions of the plurality of microreactions;
- c) using a first parameter slider by a user to select a population of preliminary positive amplifications that satisfy the first parameter requirement;
- d) using a second parameter slider by a user to remove false positive amplifications that satisfy the second parameter requirement from the population of preliminary positive amplifications;
- and
- e) counting positive amplifications with the false positive amplifications removed as final positive amplifications.
3 more elements
Dependencies are inferred from the claim text.