Antibodies that bind to natively folded myocilin
AssigneesGeorgia Tech Research Corporation, Board of Regents, The University of Texas System
InventorsRaquel L. Lieberman, Shannon E. Hill, Athena Patterson-Orazem, Jennifer A. Maynard, Ahlam Qerqez, Laura Azouz Yuan
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Abstract
Myocilin-binding agents including antibodies and antigen binding fragments thereof and fusion proteins that immunospecifically bind the coiled-coil domain of myocilin but do not bind to misfolded myocilin are provided herein. The disclosed antibodies and antigen binding fragments and fusion proteins are useful for the detection and extraction of natively folded myocilin from a sample.
Claims18
An antibody or antigen-binding fragment thereof that specifically binds to natively folded myocilin but does not bind to misfolded myocilin, wherein the antibody comprises the light chain variable region (LCVR) having at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity to the amino acid sequence according to SEQ ID NO:7, and the heavy chain variable region (HCVR) having at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity to the amino acid sequence according to SEQ ID NO:8.
An antibody or antigen-binding fragment thereof that specifically binds to natively folded myocilin, wherein the antibody comprises (a) an HCDR1 domain having an amino acid sequence according to SEQ ID NO:12;
- (b) an HCDR2 domain having an amino acid sequence selected from the group consisting of SEQ ID NOs:13, 18, and 42-45;
- (c) an HCDR3 domain having the amino acid sequence FVY;
- (d) an LCDR1 domain having an amino acid sequence according to SEQ ID NO:9;
- (e) an LCDR2 domain having an amino acid sequence according to SEQ ID NO:10;
- and (f) an LCDR3 domain having an amino acid sequence selected from the group consisting of SEQ ID NO:11, 14-17 and 19-39.
A myocilin fusion protein comprising the amino acid sequence of any one of SEQ ID NO:1-8 or a functional variant thereof linked to an immunoglobulin domain, wherein the fusion protein binds myocilin.
Dependencies are inferred from the claim text.