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US 2022/0026435 A1Published application
Kits and methods for quantifying collagen
AssigneeAvicenna Nutraceutical, LLC
InventorsAli ELNAJJAR, Ali MOURAD, Mark Ernst BRANDT, Alexander Joseph DEPTULA
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Abstract
Disclosed herein are kits and methods for quantifying the amount of collagen in a test sample. The kits and methods described involve the use of undenatured type II collagen as a standard for producing accurate and reproducible results with respect to quantifying the amount of collagen in a sample used in cosmetics, food products, and pharmaceuticals or nutritional supplements.
Claims56
A kit comprising
- (a) an oxidant;
- (b) a chromophore;
- and
- (c) undenatured type II collagen.
1 more element
A method for quantifying the amount of type II collagen present in a sample, the method comprising
- (a) hydrolyzing the sample with an acid to produce a second sample comprising hydroxyproline;
- (b) admixing an oxidant with the second sample to produce a third sample;
- (c) admixing a chromophore with the third sample to produce a fourth sample;
- (d) measuring the absorbance of the fourth sample at a specific wavelength between 500 nm to 600 nm;
- and
- (e) correlating the absorbance of the fourth sample to a standard curve of absorbance vs. concentration of type II collagen.
3 more elements
Dependencies are inferred from the claim text.