System and method for analyzing extracellular vesicles with an optical biosensor

A system for analyzing extracellular vesicles comprising:

• an optical waveguide biosensor;
• extracellular vesicles bound to the optical waveguide biosensor;
• and
• a ligand bound to the extracellular vesicles, the ligand including a label.

A system for analyzing extracellular vesicles comprising:

• a microplate including a plurality of wells;
• an optical waveguide biosensor positioned in each of the plurality of wells;
• and
• a sample positioned in each of the plurality of wells;
• wherein the samples each include the intravesicular content of ruptured extracellular vesicles that were bound to the optical waveguide biosensors.

A kit for analyzing extracellular vesicles comprising:

• a microplate including a plurality of wells;
• an optical waveguide biosensor positioned in each of the plurality of wells;
• binding agents bound to each of the optical waveguide biosensors, the binding agents being configured to bind to extracellular vesicles;
• and
• at least one of:
• (a) ligands configured to bind to the extracellular vesicles, the ligands including a label;
• or
• (b) a reagent configured to rupture the extracellular vesicles.