Methods to detect trace levels of genetic materials using colloidal gold nanoparticles on quartz paper or metamaterial substrates and surface-enhanced raman scattering
AssigneeSalvo Technologies, Inc.
InventorsAnne-Marie Dowgiallo, Hugh Garvey, John Dougherty
Life of this patent
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Abstract
The use of colloidal gold nanoparticles deposited on quartz paper or metamaterial substrates to enable trace level detection of biological materials such as genetic materials using Surface-enhanced Raman Scattering (SERS) wherein this molecule of interest may be tested in pure solutions or human blood is disclosed.
Claims9
A method of Surface Enhanced Raman Scattering testing for 1 to 10 ppm analyte using refrigerated 1 mL solutions of prepared colloidal gold nanoparticles having a position of the surface plasmon peak at 540 nm and Phenylalanine or other analytes of interest testing in blood on paper substrate is accomplished as follows:
- creating a mixture by mixing either by hand or vortex for a few seconds 20-30 μL of said prepared colloidal gold nanoparticles with 20-30 μL of said analyte dissolved in blood at a concentration <60 mg/dL and 0.6-1 μL 20 mM sodium chloride (NaCl);
- and,
- then pipetting 15-20 μL of said mixture onto an 8 mm quartz paper circle and measuring immediately with said Surface Enhanced Raman Scattering system at a power density of approximately 100 W/cm2.
A method of Surface Enhanced Raman Scattering testing for 1 to 10 ppm analyte using refrigerated 1 mL solutions of prepared colloidal gold nanoparticles having a position of the surface plasmon peak at 540 nm and phenylalanine or other analytes of interest testing on metamaterial is accomplished as follows:
- mixing either by hand or vortex for a few seconds 20-30 μL of said prepared colloidal gold nanoparticles with 20-30 μL of said analyte dissolved in pure solvent at a concentration <60 mg/dL and 0.6-1 μL 20 mM sodium chloride (NaCl);
- and,
- then pipetting 5-10 μL of the mixture onto 4 mm metamaterial substrate and allowing said mixture to dry before interrogating with 785 nm laser excitation and Raman instrumentation at a power density of approximately 100 W/cm2.
A method of Surface Enhanced Raman Scattering testing for 1 to 10 ppm analyte using refrigerated 1 mL solutions of prepared colloidal gold nanoparticles having a position of the surface plasmon peak at 540 nm and said analyte testing on metamaterial in blood is accomplished as follows:
- mixing 20-30 μL of said prepared colloidal gold nanoparticles with 0.6-1 μL 20 mM NaCl and 5 μL of this mixture is pipetted onto a metamaterial substrate and allowed to dry;
- placing a non-metalized porous metamaterial substrate on top of said metallized metamaterial substrate containing aggregated colloidal gold nanoparticles as a filter for the red blood cells;
- then dissolving 10 μL of said analyte of interest in blood at a concentration <60 mg/dL creating a solution and pipetting said solution on top of the non-metalized porous metamaterial substrate;
- drying said solution on said sandwiched metamaterial substrate and non-metalized porous metamaterial substrate metamaterials before removing a top filter substrate from said non-metalized porous metamaterial substrate;
- and,
- then interrogating the sample with 785 nm laser excitation and Raman instrumentation at a power density of approximately 100 W/cm2.
Dependencies are inferred from the claim text.