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US 2022/0026362 A1Published application
Method for Correcting Interference of Multicolor Fluorescence Channels
OwnerYan Wang, Cory McCluskeyinventors
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OwnerYan Wang, Cory McCluskeyinventors
Estimating the term…
Provided is a fluorescence reader that uses two excitation channels and can read up to seven different fluorescent dyes in a single run. Each excitation channel has one light source and one single excitation filter and one dichroic mirror. One excitation channel is capable of exciting multiple fluorescent dyes and can be used to distinguish multiple dyes in combination with multiple emission filters. The excitation channels are driven by a motor that can automatically switch the two excitation channels for taking images of up to seven different fluorescent dyes. An algorithm to calibrate the crosstalk between different fluorescent dyes is also provided. Also provided is a method for analyzing digital PCR data using a ratio of two fluorescence emission readings.
A method for correcting bleed-through from n different fluorescence channels into fluorescence channel A in multicolor fluorescence recordings, wherein corrected fluorescence intensity of fluorescence channel A is calculated as following:
The method of claim 11, wherein the method for determining a calibration constant (Ki) for correcting bleed-through from ith fluorescence channel to fluorescence channel A comprises the steps of:
Dependencies are inferred from the claim text.