Method and system for optical force measurement

A method for determining an activity of an enzyme that is interacting with at least part of a structure, the method comprising:

• controlling a light-sensitive system to determine light information based on light from the structure, the light being incident on at least part of the light-sensitive system, wherein the at least part of the structure comprises one or more optically active entities, wherein at least one of (i) an optical activity of the one or more optically active entities and (ii) a quantity of the one or more optically active entities depends on a tension in the at least part of the structure, the tension being generated by enzymatic reactions involving the enzyme, and wherein the light information defines a light property value associated with the at least part of the structure;
• and
• determining the activity of the enzyme on the basis of the light property value.

A controller configured to perform steps of:

• controlling a light-sensitive system to determine light information based on light from a structure, the light being incident on at least part of the light-sensitive system, wherein at least part of the structure comprises one or more optically active entities, wherein at least one of (i) an optical activity of the one or more optically active entities and (ii) a quantity of the one or more optically active entities depends on a tension in the at least part of the structure, the tension being generated by enzymatic reactions involving an enzyme, and wherein the light information defines a light property value associated with the at least part of the structure;
• and
• determining the activity of the enzyme on the basis of said light property value.

A non-transitory computer-readable storage medium comprising instructions which, when executed by a computer, cause the computer to:

• determine an activity of an enzyme that is interacting with at least part of a structure on the basis of a light property value associated with the at least part of the structure, the light property value being defined by light information, the light information being determined by a light-sensitive system based on light from the structure incident on at least part of the light-sensitive system, wherein:
• the at least part of the structure comprises one or more optically active entities, wherein at least one of (i) an optical activity of the one or more optically active entities and (ii) a quantity of the one or more optically active entities depends on a tension in the at least part of the structure, the tension being generated by enzymatic reactions involving the enzyme.

A method for causing at least part of a structure to exhibit a tension-dependent optical activity for enabling determination of an activity of an enzyme that is interacting with the at least part of the structure, the method comprising combining the at least part of the structure with a fluid and one or more optically active entities, wherein the one or more optically active entities in the fluid can bind to the at least part of the structure and wherein at least one of:

• a binding property of the one or more optically active entities in respect of the at least part of the structure depends on tension generated in the at least part of the structure by enzymatic reactions involving the enzyme;
• and
• an optical property of the one or more optically active entities bound to the at least part of the structure depends on tension generated in the at least part of the structure by enzymatic reactions involving the enzyme.