Method and kit for detecting mycobacterium tuberculosis
AssigneeDelta Electronics, Inc.
InventorsYi-Chen Li, Chih-Cheng Tsou, Min-Hsien Wu, Hsin-Yao Wang, Chien-Ru Lin
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Abstract
A method and a kit for detecting Mycobacterium tuberculosis are provided. The method includes a step of performing a nested qPCR assay to a specimen. The nested qPCR assay includes a first round of amplification using external primers and a second round of amplification using internal primers and a probe. The external primers have sequences of SEQ ID NOs. 1 and 2, and the internal primers and the probe have sequences of SEQ ID NOs. 3 to 5.
Claims13
A method for detecting Mycobacterium tuberculosis, comprising a step of performing a nested qPCR assay to a specimen, wherein the nested qPCR assay comprises a first round of amplification using external primers and a second round of amplification using internal primers and a probe, the external primers have sequences of SEQ ID NOs. 1 and 2, and the internal primers and the probe have sequences of SEQ ID NOs. 3 to 5.
A method for detecting Mycobacterium tuberculosis, comprising steps of:
- performing a screening by qPCR to a specimen using primers and a probe having sequences of SEQ ID NOs. 3 to 5;
- and
- performing a nested qPCR assay to the specimen having a positive result in the screening, wherein the nested qPCR assay comprises a first round of amplification using external primers and a second round of amplification using internal primers and a probe, the external primers have sequences of SEQ ID NOs. 1 and 2, and the internal primers and the probe have sequences of SEQ ID NOs. 3 to 5.
A kit for detecting Mycobacterium tuberculosis, comprising external primers, internal primers and a probe, wherein the external primers have sequences of SEQ ID NOs. 1 and 2, the internal primers have sequences of SEQ ID NOs. 3 and 4, and the probe has a sequence of SEQ ID NO. 5.
Dependencies are inferred from the claim text.