Improved Filamentous Fungal Host Cells

22:

• The variant Ire1 polypeptide of claim 21, wherein the substitution at position 153 or a position corresponding to position 153 is with Thr.

23:

• The variant Ire1 polypeptide of claim 21, wherein an Ala at position 153 or a position corresponding to position 153 is substituted with Thr.

24:

• The variant Ire1 polypeptide of claim 21, which further comprises a Thr at position 150 of the Trichoderma reesei Ire1 polypeptide of SEQ ID NO:
• 2 or a Thr at a position corresponding to position 150 of the Trichoderma reesei Ire1 of SEQ ID NO:
• 2.

26:

• The polynucleotide of claim 25, wherein the substitution at position 153 or a position corresponding to position 153 is with Thr.

27:

• The polynucleotide of claim 25, wherein an Ala at position 153 or a position corresponding to position 153 is substituted with Thr.

28:

• The polynucleotide of claim 25, wherein the variant Ire1 polypeptide further comprises a Thr at position 150 of the Trichoderma reesei Ire1 polypeptide of SEQ ID NO:
• 2 or a Thr at a position corresponding to position 150 of the Trichoderma reesei Ire1 of SEQ ID NO:
• 2.

29:

• A nucleic acid construct comprising the polynucleotide of claim 25.

31:

• The recombinant filamentous fungal host cell of claim 30, wherein the substitution at position 153 or a position corresponding to position 153 is with Thr.

32:

• The recombinant filamentous fungal host cell of claim 30, wherein an Ala at position 153 or a position corresponding to position 153 is substituted with Thr.

33:

• The recombinant filamentous fungal host cell of claim 30, wherein the variant Ire1 polypeptide further comprises a Thr at position 150 of the Trichoderma reesei Ire1 polypeptide of SEQ ID NO:
• 2 or a Thr at a position corresponding to position 150 of the Trichoderma reesei Ire1 of SEQ ID NO:
• 2.

34:

• The recombinant filamentous fungal host cell of claim 30, which is of a genus selected from the group consisting of Acremonium, Aspergillus, Aureobasidium, Bjerkandera, Ceriporiopsis, Chrysosporium, Coprinus, Coriolus, Cryptococcus, Filibasidium, Fusarium, Humicola, Magnaporthe, Mucor, Myceliophthora, Neocallimastix, Neurospora, Paecilomyces, Penicillium, Phanerochaete, Phlebia, Piromyces, Pleurotus, Schizophyllum, Talaromyces, Thermoascus, Thielavia, Tolypocladium, Trametes and Trichoderma.

37:

• The method of claim 36, wherein the substitution at position 153 or a position corresponding to position 153 is with Thr.

38:

• The method of claim 36, wherein an Ala at position 153 or a position corresponding to position 153 is substituted with Thr.

39:

• The method of claim 36, wherein the variant Ire1 polypeptide further comprises a Thr at position 150 of the Trichoderma reesei Ire1 polypeptide of SEQ ID NO:
• 2 or a Thr at a position corresponding to position 150 of the Trichoderma reesei Ire1 of SEQ ID NO:
• 2.

40:

• The method of claim 36, wherein the filamentous fungal host cell is of a genus selected from the group consisting of Acremonium, Aspergillus, Aureobasidium, Bjerkandera, Ceriporiopsis, Chrysosporium, Coprinus, Coriolus, Cryptococcus, Filibasidium, Fusarium, Humicola, Magnaporthe, Mucor, Myceliophthora, Neocallimastix, Neurospora, Paecilomyces, Penicillium, Phanerochaete, Phlebia, Piromyces, Pleurotus, Schizophyllum, Talaromyces, Thermoascus, Thielavia, Tolypocladium, Trametes and Trichoderma.