Phytase mutants
AssigneeQingdao Vland Biotech Group Co., Ltd.
InventorsXiuxiu WU, Huaming Wang
Life of this patent
Estimating the term…
Abstract
Provided are mutants PHY1, PHY4 and PHY5 of a wild-type phytase APPA. After being treated for 10 min at 80° C., the residual enzyme activities of the mutants PHY1, PHY4 and PHY5 are respectively higher by 33.85%, 53.11% and 75.86% compared with that of APPA-M; after being treated for 5 min at 85° C., the residual enzyme activities of the mutants PHY1, PHY4 and PHY5 are respectively higher by 14.89%, 28.45% and 44.94% compared with that of APPA-M, and the heat resistance of these mutants is significantly higher than that of APPA-M.
Claims10
A phytase mutant, comprising the amino acid sequence shown in (I) or (II):
- (I) an amino acid sequence which has at least 70% identity to the amino acid sequence of SEQ ID NO:
- 1;
- (II) an amino acid sequence which has at least one immune epitope of the phytase, and comprises a modification, substitution, deletion, and/or insertion of one or more amino acids within the amino acid sequence of SEQ ID NO:1, wherein 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16 or 17 amino acids are substituted within the amino acid sequence of SEQ ID NO:1.
Dependencies are inferred from the claim text.