Variants of CRISPR from Prevotella and Francisella 1 (Cpf1)
AssigneeThe General Hospital Corporation
InventorsJ. Keith Joung, Benjamin Kleinstiver
Life of this patent
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Abstract
Engineered CRISPR from Prevotella and Francisella 1 (Cpf1) nucleases with altered and improved target specificity and their use in genomic engineering, epigenomic engineering, genome targeting, genome editing, and in vitro diagnostics.
Claims28
1-30. (canceled)
An isolated CRISPR from Prevotella and Francisella 1 (Cpf1) protein, wherein the protein is from Lachnospiraceae bacterium ND2006 (LbCpf1), comprising a sequence that is at least 80% identical to the amino acid sequence of amino acids 19-1246 of SEQ ID NO:1, with mutations at one or more of the following positions:
- S202, N274, N278, K290, K367, K532, K609, K915, Q962, K963, K966, K1002 and/or S1003 of amino acids 1-1228 of SEQ ID NO:10.
An isolated nucleic acid encoding an isolated Cpf1 protein, wherein the protein is from LbCpf1, comprising a sequence that is at least 80% identical to the amino acid sequence of amino acids 19-1246 of SEQ ID NO:1, with mutations at one or more of the following positions:
- S202, N274, N278, K290, K367, K532, K609, K915, Q962, K963, K966, K1002 and/or S1003 of amino acids 1-1228 of SEQ ID NO:10.
Dependencies are inferred from the claim text.