Optimized protein linkers and methods of use
AssigneePairwise Plants Services, Inc.
InventorsSharon Leigh Guffy, Joseph Matthew Watts
Life of this patent
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Abstract
The invention relates to peptide linkers and fusion proteins comprising linkers designed for optimizing the activity of the proteins comprised therein, and methods for using the same. The invention further relates to newly designed Cas12a-based adenine base editors.
Claims24
1-72. (canceled)
A polypeptide comprising any one of the amino acid sequences of SEQ ID NOs:
- 1-24.
A polypeptide comprising a Cas12a domain linked to any one of the amino acid sequences of SEQ ID NOs:
- 1-24.
A fusion protein comprising a Cas12a domain, a polypeptide of interest and any one of the amino acid sequences of SEQ ID NOs:
- 1-24.
An expression cassette or vector comprising a polynucleotide encoding a fusion protein comprising a Cas12a domain and a polypeptide of interest, wherein the Cas12a domain and the polypeptide of interest are linked to one another via any one of the amino acid sequences of SEQ ID NOs:
- 1-24.
Dependencies are inferred from the claim text.