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AssigneesKataoka Corporation, Kyoto Prefectural Public University Corporation
InventorsPing DAI, Yukimasa TAKEDA, Yoshinori HARADA, Junichi MATSUMOTO, Ayumi KUSAKA
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It is a main object of the present invention to provide a new producing method capable of efficiently performing direct conversion or induction from a somatic cell to an insulin-producing cell. The present invention can include, for example, a process for producing an insulin-producing cell by direct differentiation induction from a somatic cell, comprising a step of culturing a somatic cell in a serum-free differentiation induction medium, or a step of culturing a somatic cell in a differentiation induction medium containing 5 μg/mL or more of insulin. According to the present invention, insulin-producing cells having a high insulin secretion ability can be produced directly and efficiently from a somatic cell. The insulin-producing cells obtained according to the present invention are useful in regenerative medicine and the like.
A process for producing an insulin-producing cell by direct differentiation induction from a somatic cell, comprising a step of culturing a somatic cell in a serum-free differentiation induction medium.
A process for producing an insulin-producing cell by direct differentiation induction from a somatic cell, comprising a step of culturing a somatic cell in a differentiation induction medium containing 5 μg/mL or more of insulin.
(canceled)
A differentiation induction composition for producing an insulin-producing cell by directly inducing differentiation from a somatic cell, wherein the composition is serum-free.
A differentiation induction composition for producing an insulin-producing cell by directly inducing differentiation from a somatic cell, wherein the composition contains 5 μg/mL or more of insulin.
Dependencies are inferred from the claim text.