Loading patent…
AssigneeThe Regents of the University of California
InventorsKyriacos A. Athanasiou, Jerry C. Hu, Wendy E. Brown
Estimating the term…
Methods and systems for enhancing cell populations such as chondrocytes for tissue engineering applications, e.g., for production of neocartilage. The methods and systems of the present invention feature the introduction of a hypotonic buffer to the cells during the cell isolation process, which results in neotissue (e.g., neocartilage) constructs that are significantly more mechanically robust as compared to those not treated with hypotonic buffer. The methods and systems may further comprise introducing cytochalasin D to cells purified with a hypotonic buffer, which can further bolster the mechanical properties and matrix deposition of the cells. The methods and systems result in neocartilage engineered from chondrocytes, for example, from fetal aged tissue, having compressive properties on par with native adult articular cartilage.
A method of enhancing a cartilage cell population, the method comprises:
A method of enhancing a human cartilage cell population, the method comprises:
A method of enhancing a non-articular cartilage cell population, the method comprises:
Dependencies are inferred from the claim text.