Methods of producing venous angioblasts and sinusoidal endothelial cell-like cells and compositions thereof
AssigneeUniversity Health Network
InventorsGordon Keller, Blair Kenneth Gage
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Abstract
Disclosed herein are methods of producing a population of venous angioblast cells from stem cells using a venous angioblast inducing media and optionally isolating a CD34+ population from the cell population comprising the venous angioblast cells, for example using a CD34 affinity reagent, CD31 affinity reagent and/or CD144 affinity reagent, optionally with or without a CD73 affinity reagent as well as methods of further differentiating the venous angioblasts in vitro to produce SEC-LCs and/or in vivo to produce SECs. Uses of the cells and compositions comprising the cells are also described.
Claims22
A method of producing sinusoidal endothelial cell-like cells (SEC-LCs) comprising:
- providing stem cells or angioblasts;
- and
- culturing the stem cells or angioblasts under conditions in which SEC-LCs are produced, wherein the conditions comprise:
- a) culturing the stem cells or angioblasts in the presence of bFGF;
- or
- b) culturing the stem cells or angioblasts in the presence of vascular endothelial growth factor (VEGF)-A to produce endothelial cells followed by culturing the endothelial cells in the presence of a TGF-beta signaling inhibitor, cyclic AMP (cAMP) signaling agonist, VEGF-C;
- or
- c) culturing the stem cells or angioblasts under hypoxic conditions, thereby producing SEC-LCs.
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Dependencies are inferred from the claim text.