Methods for generating, evaluating, gene editing and cloning pluripotent stem cells comprising a lethal haplotype
AssigneeInguran, LLC
InventorsDavid Kendall, Diego Moreno
Life of this patent
Estimating the term…
Abstract
The invention includes a method of evaluating a bovine embryo by fertilizing an egg obtained from a first bovine heterozygote of a recessive lethal haplotype with sperm cells obtained from a second bovine heterozygote of the recessive lethal haplotype; producing the embryo from the fertilized egg, wherein the embryo is homozygous for the lethal haplotype; establishing a cell culture from the embryo; collecting a plurality of cultured cells; and obtaining omics data, comprising one or more features, from the plurality of cultured cells.
Claims15
A method of evaluating a bovine embryo comprising:
- fertilizing an egg obtained from a first bovine heterozygote of a recessive lethal haplotype with sperm cells obtained from a second bovine heterozygote of the recessive lethal haplotype;
- producing the embryo from the fertilized egg, wherein the embryo is homozygous for the lethal haplotype;
- establishing a cell culture from the embryo;
- collecting a plurality of cultured cells;
- and
- obtaining omics data, comprising one or more features, from the plurality of cultured cells.
A method of evaluating a plurality of bovine embryos comprising:
- fertilizing a plurality of eggs obtained from one or more female bovine heterozygotes of a recessive lethal haplotype with sperm cells obtained from one or more male bovine heterozygotes of the recessive lethal haplotype;
- producing the plurality of embryos from the plurality of fertilized eggs, wherein the embryos are homozygous for the lethal haplotype;
- identifying a first group of one or more embryos from the plurality of embryos that are underdeveloped or dead after a first period of time;
- obtaining omics data from the identified first group.
Dependencies are inferred from the claim text.