Trituration devices for tissue disaggregation

A trituration device for tissue disaggregation, the device comprising:

• a device housing;
• a rotor block positioned in the device housing, the rotor block including a central channel and at least one rotor blade;
• a trituration grater positioned within the device housing to contact the rotor block, the trituration grater including multiple angled cutting holes;
• a collection vessel positioned at an end of the device housing to collect disaggregated tissue of a tissue specimen;
• and
• an actuating stem including a first end, a second end opposite the first end, and a middle portion defined between the first end and the second end, wherein:
• a cross-sectional width of the middle portion is greater than a cross-sectional width of the first end and the second end;
• the actuating stem extends through the central channel of the rotor block, and a shape of the central channel of the rotor block corresponds to a shape of the cross-section of the middle portion of the actuating stem to facilitate rotation of the rotor block in response to rotation of the actuating stem;
• the collection vessel includes a central column defining an opening;
• and
• the second end of the actuating stem is received in the opening of the central column.

A method of disaggregating tissue using a trituration device, the device including a device housing, a rotor block comprising a solid cylinder including a central channel and at least one recess that defines a rotor blade, a trituration grater including multiple angled cutting holes, a collection vessel to collect disaggregated tissue, and an actuating stem, the method comprising:

• positioning a tissue specimen within the at least one recess of the rotor block;
• inserting the rotor block into the device housing to contact the trituration grater, while the tissue specimen remains in the at least one recess of the rotor block;
• inserting the actuating stem through the central channel of the rotor block;
• and
• rotating the actuating stem using a motor or crank to rotate the rotor block and press the tissue specimen against the trituration grater to receive disaggregated tissue in the collection vessel.