Sieve system and methods for cell media exchange
AssigneeThe Regents of the University of California
InventorLawrence Kulinsky
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Abstract
Methods and systems for cell media exchange wherein media may be aspirated without also aspirating cells and wherein removal and re-plating of cells is not necessarily required. For example, the cell culture system or apparatus features a gap that is small enough to retain cells therein and also sized to prevent media from leaking. The methods and systems of the present invention may help reduce stress and damage to cells as compared to traditional methods that require removing and re-plating cells.
Claims20
A cell culture sieve system (100) comprising:
- a. a culture substrate (105);
- b. a side wall (110) disposed atop the culture substrate (105) to form a container for holding media and cells in culture;
- c. a gap (122) disposed in the side wall (110) to directly connect an interior of the container with an exterior of the cell culture sieve system, wherein the gap (122) is configured to retain media via surface tension and to prevent media from leaking from the container;
- and
- d. a slot (128) disposed between a bottom of the side wall (110) and the culture substrate (105) such that the slot (128) is fluidly connected with the gap (122), wherein the slot (128) is sized and configured to receive a pipette tip (132);
- wherein when suction is applied to the gap (122) via a pipette tip (132) positioned within the gap (122) media is aspirated from the container.
A cell culture sieve system (100) comprising:
- a. a culture substrate (105);
- b. a side wall (110) disposed atop the culture substrate (105) to form a container for holding media and cells in culture;
- c. a gap (122) disposed in the side wall (110) to directly connect an interior of the container with an exterior of the cell culture sieve system;
- and
- d. an unobstructed pipette positioning area (136), such that a pipette (130) may be positioned with its tip (132) in contact with the side wall (110) at an angle such that the tip (132) and the side wall (110) form a seal.
A cell culture sieve system (100) comprising:
- a. a culture substrate (105);
- b. a side wall (110) disposed atop the culture substrate (105) to form a container for holding media and cells in culture;
- c. a gap (122) disposed in the side wall (110) to directly connect an interior of the container with an exterior of the cell culture sieve system;
- and
- d. an aspiration socket (134) disposed in the side wall (110) around the gap so as to guide positioning of a pipette tip (132) to form a seal between the tip (132) and the side wall (110) such that media may be aspirated through the gap (122) via the pipette tip (132).
Dependencies are inferred from the claim text.