Recombinant antibody of anti-human n-terminal pro-brain natriuretic peptide

An isolated binding protein comprising an antigen-binding domain, wherein the antigen-binding domain comprises at least one complementarity determining region (CDR) selected from the following amino acid sequences:

• or has at least 80% sequence identity with the complementarity determining region of the following amino acid sequences and has an affinity of KD≤2.26×10−8 to a N-terminal pro-brain natriuretic peptide (NT-proBNP);
• the complementarity determining region CDR-VH1 is G-X1-S-X2-T-T-Y-Y-X3-D, wherein
• X1 is F or P, X2 is I, V or L, and X3 is I, V or L;
• the complementarity determining region CDR-VH2 is M-T-K-D-X1-N-A-V-H-X2-P-T-X3-R-S, wherein
• X1 is G or A, X2 is Q or N, and X3 is I, V or L;
• the complementarity determining region CDR-VH3 is V-X1-G-X2-1-D-X3-G, wherein
• X1 is R or K, X2 is I, V or L, and X3 is F or W;
• the complementarity determining region CDR-VL1 is G-S-S-D-X1-V-G-X2-G-D-Y-X3-N, wherein
• X1 is Q or N, X2 is F or P, and X3 is I, V or L;
• the complementarity determining region CDR-VL2 is I-F-X1-A-X2-S-R-X3-R-G, wherein
• X1 is G or A, X2 is T, Y or S, and X3 is 1, V or L;
• the complementarity determining region CDR-VL3 is G-S-X1-N-S-R-X2-Y-V-X3-G, wherein
• X1 is P, A or G, X2 is GG or N, and X3 is F or W;
• preferably:
• X1 is F in the complementarity determining region CDR-VH1;
• X1 Is G in the complementarity determining region CDR-VH2;
• X1 is R in the complementarity determining region CDR-VH3;
• X2 is F in the complementarity determining region CDR-VL1;
• X1 is G in the complementarity determining region CDR-VL2;
• X3 is F in the complementarity determining region CDR-VL3;
• preferably, X2 is I in the complementarity determining region CDR-VH1;
• preferably, X2 is V in the complementarity determining region CDR-VH1;
• preferably, X2 is L in the complementarity determining region CDR-VH1;
• preferably, X3 is I in the complementarity determining region CDR-VH1;
• preferably, X3 is V in the complementarity determining region CDR-VH1;
• preferably, X3 is L in the complementarity determining region CDR-VH1;
• preferably, X2 is Q in the complementarity determining region CDR-VH2;
• preferably, X2 is N in the complementarity determining region CDR-VH2;
• preferably, X3 is I in the complementarity determining region CDR-VH2;
• preferably, X3 is V in the complementarity determining region CDR-VH2;
• preferably, X3 is L in the complementarity determining region CDR-VH2;
• preferably, X2 is I in the complementarity determining region CDR-VH3;
• preferably, X2 is V in the complementarity determining region CDR-VH3;
• preferably, X2 is L in the complementarity determining region CDR-VH3;
• preferably, X3 is F in the complementarity determining region CDR-VH3;
• preferably, X3 is W in the complementarity determining region CDR-VH3;
• preferably, X1 is Q in the complementarity determining region CDR-VL1;
• preferably, X1 is N in the complementarity determining region CDR-VL1;
• preferably, X3 is I in the complementarity determining region CDR-VL1;
• preferably, X3 is V in the complementarity determining region CDR-V 1;
• preferably, X3 is L in the complementarity determining region CDR-V 1;
• preferably, X2 is T in the complementarity determining region CDR-VL2;
• preferably, X2 is Y in the complementarity determining region CDR-VL2;
• preferably, X2 is S in the complementarity determining region CDR-VL2;
• preferably, X3 is I in the complementarity determining region CDR-VL2;
• preferably, X3 is V in the complementarity determining region CDR-VL2;
• preferably, X3 is L in the complementarity determining region CDR-VL2;
• preferably, X1 is P in the complementarity determining region CDR-VL3;
• preferably, X1 is A in the complementarity determining region CDR-VL3;
• preferably, X1 is G in the complementarity determining region CDR-VL3;
• preferably, X2 is GG in the complementarity determining region CDR-VL3;
• preferably, X2 is N in the complementarity determining region CDR-VL3.