Hydrogel-forming peptides, and methods of use thereof
AssigneeNanyang Technological University
InventorsAli Gilles Tchenguise MISEREZ, Shu Hui HIEW, Chor Yong TAY, Jun Kit WANG
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Abstract
The present invention, as disclosed herein, provides an isolated peptide, and a composition or material comprising a hydrogel, for the delivery of an active agent. The hydrogel comprises one or more isolated peptides and an active agent encapsulated in the hydrogel. The hydrogel is at least partially in a β-sheet conformation. Further provided are a method for the encapsulation of an active agent in a hydrogel, a method for treating or diagnosing a condition or disease in a subject in need thereof.
Claims20
An isolated peptide comprising or consisting of the amino acid sequence:
- (SEQ ID NO:
- 3)
- (GX1Z1GGZ2GB)n(X2)m(GX1Z1GGZ2GB)o
- wherein
- each B is independently valine (V) or isoleucine (I);
- each X1 independently is an aliphatic amino acid, preferably leucine (L);
- each Z1, Z2 is independently an aromatic amino acid, preferably tyrosine (Y);
- X2 is any amino acid;
- m is 0 or an integer from 1 to 10;
- n and o are independently 0 or an integer selected from 1, 2 or 3, provided that n+o is at least 1;
- and
- wherein the isolated peptide is up to 50 amino acids in length.
A method for the encapsulation of an active agent in a hydrogel, the method comprising:
- (i) providing an aqueous solution of hydrogel-forming peptides, wherein the hydrogel-forming peptides comprises one or more isolated peptides, the one or more isolated peptides comprising or consisting the amino acid sequence:
- (GX1Z1GGZ2GB)n(X2)m(GX1Z1GGZ2GB)o (SEQ ID NO:
- 3)
- wherein
- each B is independently valine (V) or isoleucine (I);
- each X1 independently is an aliphatic amino acid, preferably leucine (L);
- each Z1, Z2 is independently an aromatic amino acid, preferably tyrosine (Y);
- X2 is any amino acid;
- m is 0 or an integer from 1 to 10;
- n and o are independently 0 or an integer selected from 1, 2 or 3, provided that n+o is at least 1;
- wherein the isolated peptide is up to 50 amino acids in length;
- (ii) combining the aqueous solution of the hydrogel-forming peptides with a solution of an active agent;
- and
- (iii) inducing formation of the hydrogel, wherein the isolated peptides in the hydrogel are at least partially in a β-sheet conformation.
A method for treating or diagnosing a condition or disease in a subject in need thereof, comprising:
- (i) administering a composition or material comprising a hydrogel to a subject, wherein the hydrogel comprises:
- a. hydrogel-forming peptides comprising one or more isolated peptides, the isolated peptides comprising or consisting the amino acid sequence:
- (GX1Z1GGZ2GB)n(X2)m(GX1Z1GGZ2GB)o (SEQ ID NO:
- 3)
- wherein
- each B is independently valine (V) or isoleucine (I);
- each X1 independently is an aliphatic amino acid, preferably leucine (L);
- each Z1, Z2 is independently an aromatic amino acid, preferably tyrosine (Y);
- X2 is any amino acid;
- m is 0 or an integer from 1 to 10;
- n and o are independently 0 or an integer selected from 1, 2 or 3, provided that n+o is at least 1;
- wherein the isolated peptide is up to 50 amino acids in length;
- wherein the isolated peptides in the hydrogel are at least partially in a β-sheet conformation, and
- b. a pharmaceutical or diagnostic agent, wherein the pharmaceutical or diagnostic agent is encapsulated in the hydrogel.
Dependencies are inferred from the claim text.