Methods for producing a rice protein peptide and applications thereof
AssigneeWuxi Jinnong Biotechnology Co., Ltd
InventorsTianxiang CHEN, Qiusheng YU, Zhenzhen XU
Life of this patent
Estimating the term…
Abstract
The present invention provides a method for producing a rice protein peptide including (1) preparing a slurry of rice residue protein, followed by sterilizing the rice residue protein; (2) first crushing; (3) undergoing a first proteolysis; (4) second crushing; (5) undergoing a second proteolysis; (6) conducting solid-liquid separation; (7) concentrating; (8) membrane filtering; (9) providing an anti-microbial treatment; (10) performing spray drying. The present method performing super-fine grinding on the proteolytic rice residue protein. During the process of second proteolysis, the protease fully contacts with the substrate, thereby promoting the proteolytic efficiency and taste.
Claims10
A method for producing a rice protein peptide, comprising:
- (1) preparing 5-15% of rice residue protein slurry, followed by sterilizing the rice residue protein slurry at a temperature of 100-135° C. for a reaction time of 5 seconds to 30 minutes to obtain a material;
- (2) crushing the material by using a colloid mill or a fine crusher to obtain a crushed rice residue protein, wherein a D50 particle size distribution of the material is reduced to 30-50 microns;
- (3) adding an alkaline protease for undergoing a first proteolysis after adjusting the temperature of the rice residue protein to 45-60° C. and the pH value to a range from 9.0-12.0, wherein the first proteolysis is performed at a temperature of 45-60° C. for a reaction time of 1-5 hours;
- (4) performing super-fine grinding on the proteolytic rice residue protein by using a micro jet or a ball mill to obtain a first proteolytic fluid, wherein a Dso particle size distribution of the crushed material is 5-10 microns;
- (5) adding a neutral protease for undergoing a second proteolysis for a reaction time of 1-5 hours after adjusting the temperature of the first proteolytic fluid to 45-60° C. and the pH value to a range from 7.5-6.5, and then performing enzyme deactivation for a reaction time of 5 seconds to 30 minutes to obtain a second proteolytic fluid;
- (6) conducting solid-liquid separation on the second proteolytic fluid;
- (7) collecting filtrate after completing said solid-liquid separation and concentrating the filtrate until reaching a solid content of 10-70%, wherein the filtrate is concentrated at a temperature of 50-90° C. under a vacuum degree of 0.06-0.1 MPa;
- (8) conducting membrane filtration of the concentrated solution to obtain a feed liquid;
- (9) providing an anti-microbial treatment of the feed liquid, wherein the condition for the treatment is at a temperature of 90° C. for a reaction time of 30 minutes;
- (10) performing spray drying on the feed liquid to obtain a rice protein peptide, wherein the spray drying conditions include an inlet air temperature of 180° C., an outlet air temperature of 85-90° C., and a water content discharged is no more than 5%.
Dependencies are inferred from the claim text.